ArticleNucleic acids research2009
Peculiarities of piRNA-mediated post-transcriptional silencing of Stellate repeats in testes of Drosophila melanogaster.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Small RNA-mediated suppression of sex chromosome meiotic conflicts during Drosophila male gametogenesis.Biochemical Society transactions · 2025Review
- Outcome prediction of microdissection testicular sperm extraction based on extracellular vesicles piRNAs.Journal of assisted reproduction and genetics · 2021Article
- Review
- Article
- Computational Identification of piRNAs Using Features Based on RNA Sequence, Structure, Thermodynamic and Physicochemical Properties.Current genomics · 2019Article
- Sex and the TEs: transposable elements in sexual development and function in animals.Mobile DNA · 2019Review
- A piRNA utilizes HILI and HIWI2 mediated pathway to down-regulate ferritin heavy chain 1 mRNA in human somatic cells.Nucleic acids research · 2018Article
- Repeated Duplication of Argonaute2 Is Associated with Strong Selection and Testis Specialization in Drosophila.Genetics · 2016Article
- The piRNA pathway is developmentally regulated during spermatogenesis in Drosophila.RNA (New York, N.Y.) · 2016Article
- The RNA surveillance complex Pelo-Hbs1 is required for transposon silencing in the Drosophila germline.EMBO reports · 2015Article
- Unique somatic and malignant expression patterns implicate PIWI-interacting RNAs in cancer-type specific biology.Scientific reports · 2015Article
- Posttranscriptional regulation of gene expression by Piwi proteins and piRNAs.Molecular cell · 2014Review
- Conditional inactivation of Miwi2 reveals that MIWI2 is only essential for prospermatogonial development in mice.Cell death and differentiation · 2014Article
- Drosophila H1 regulates the genetic activity of heterochromatin by recruitment of Su(var)3-9.Science (New York, N.Y.) · 2013Article
- Germ granules in spermatogenesis of Drosophila: Evidences of contribution to the piRNA silencing.Communicative & integrative biology · 2012Article
- The "Special" crystal-Stellate System in Drosophila melanogaster Reveals Mechanisms Underlying piRNA Pathway-Mediated Canalization.Genetics research international · 2012Article
- Separation of stem cell maintenance and transposon silencing functions of Piwi protein.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Functional specialization of Piwi proteins in Paramecium tetraurelia from post-transcriptional gene silencing to genome remodelling.Nucleic acids research · 2011Article
- Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis.RNA (New York, N.Y.) · 2010Article
- piRNAs mediate posttranscriptional retroelement silencing and localization to pi-bodies in the Drosophila germline.The Journal of cell biology · 2009Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Silencing of Stellate genes in Drosophila melanogaster testes is caused by antisense piRNAs produced as a result of transcription of homologous Suppressor of Stellate (Su(Ste)) repeats. Mechanism of piRNA-dependent Stellate repression remains poorly understood. Here, we show that deletion of Su(Ste) suppressors causes accumulation of spliced, but not nonspliced Stellate transcripts both in the nucleus and cytoplasm, revealing post-transcriptional degradation of Stellate RNA as the predominant mechanism of silencing. We found a significant amount of Su(Ste) piRNAs and piRNA-interacting protein Aubergine (Aub) in the nuclear fraction. Immunostaining of isolated nuclei revealed co-localization of a portion of cellular Aub with the nuclear lamina. We suggest that the piRNA-Aub complex is potentially able to perform Stellate silencing in the cell nucleus. Also, we revealed that the level of the Stellate protein in Su(Ste)-deficient testes is increased much more dramatically than the Stellate mRNA level. Similarly, Su(Ste) repeats deletion exerts an insignificant effect on mRNA abundance of the Ste-lacZ reporter, but causes a drastic increase of beta-gal activity. In cell culture, exogenous Su(Ste) dsRNA dramatically decreases beta-gal activity of hsp70-Ste-lacZ construct, but not its mRNA level. We suggest that piRNAs, similarly to siRNAs, degrade only unmasked transcripts, which are accessible for translation.
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