ArticleNucleic acids research2009
Phosphate control over nitrogen metabolism in Streptomyces coelicolor: direct and indirect negative control of glnR, glnA, glnII and amtB expression by the response regulator PhoP.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 124 citations in OpenAlex.
- Regulatory and metabolic control of microbial biosynthetic gene clusters.Communications biology · 2026Review
- Optimization of Fermentation and Transcriptomic Analysis of a High-Protein-ProducingFoods (Basel, Switzerland) · 2026Article
- Defining the networks that connect RNase III and RNase J-mediated regulation of primary and specialized metabolism inJournal of bacteriology · 2025Article
- Structural insights into transcription regulation of the global OmpR/PhoB family regulator PhoP from Mycobacterium tuberculosis.Nature communications · 2025Article
- Orphan response regulator NnaR is critical for nitrate and nitrite assimilation inFrontiers in cellular and infection microbiology · 2024Article
- Research progress on GlnR-mediated regulation in Actinomycetes.Frontiers in microbiology · 2023Review
- Two-Component Systems ofInternational journal of molecular sciences · 2022Review
- Comparative Proteomic Analysis of Transcriptional and Regulatory Proteins Abundances inInternational journal of molecular sciences · 2022Article
- Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity.Medical sciences (Basel, Switzerland) · 2022Review
- PhoP- and GlnR-mediated regulation of metK transcription and its impact upon S-adenosyl-methionine biosynthesis in Saccharopolyspora erythraea.Microbial cell factories · 2022Article
- Effects of dual deletion of glnR and mtrA on expression of nitrogen metabolism genes in Streptomyces venezuelae.Microbial biotechnology · 2022Article
- The Global Regulator PhoU Positively Controls Growth and Butenyl-Spinosyn Biosynthesis inFrontiers in microbiology · 2022Article
- Deletion of the Response Regulator PhoP Accelerates the Formation of Aerial Mycelium and Spores inFrontiers in microbiology · 2022Article
- Multi-omics Study of Planobispora rosea, Producer of the Thiopeptide Antibiotic GE2270A.mSystems · 2021Article
- Article
- Modulation of Gene Expression in Actinobacteria by Translational Modification of Transcriptional Factors and Secondary Metabolite Biosynthetic Enzymes.Frontiers in microbiology · 2021Review
- Expression of genes of the Pho regulon is altered in Streptomyces coelicolor.Scientific reports · 2020Article
- Two-Component-System RspA1/A2-Dependent Regulation on Primary Metabolism inFrontiers in microbiology · 2020Article
- Phosphate regulator PhoP directly and indirectly controls transcription of the erythromycin biosynthesis genes in Saccharopolyspora erythraea.Microbial cell factories · 2019Article
- Structural and biochemical analysis of a phosin from Streptomyces chartreusis reveals a combined polyphosphate- and metal-binding fold.FEBS letters · 2019Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial growth requires equilibrated concentration of C, N and P sources. This work shows a phosphate control over the nitrogen metabolism in the model actinomycete Streptomyces coelicolor. Phosphate control of metabolism in Streptomyces is exerted by the two component system PhoR-PhoP. The response regulator PhoP binds to well-known PHO boxes composed of direct repeat units (DRus). PhoP binds to the glnR promoter, encoding the major nitrogen regulator as shown by EMSA studies, but not to the glnRII promoter under identical experimental conditions. PhoP also binds to the promoters of glnA and glnII encoding two glutamine synthetases, and to the promoter of the amtB-glnK-glnD operon, encoding an ammonium transporter and two putative nitrogen sensing/regulatory proteins. Footprinting analyses revealed that the PhoP-binding sequence overlaps the GlnR boxes in both glnA and glnII. 'Information theory' quantitative analyses of base conservation allowed us to establish the structure of the PhoP-binding regions in the glnR, glnA, glnII and amtB genes. Expression studies using luxAB as reporter showed that PhoP represses the above mentioned nitrogen metabolism genes. A mutant deleted in PhoP showed increased expression of the nitrogen metabolism genes. The possible conservation of phosphate control over nitrogen metabolism in other microorganisms is discussed.
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