ArticleThe Journal of biological chemistry2009
Maintenance of RNA-DNA hybrid length in bacterial RNA polymerases.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Structural basis for control of bacterial RNA polymerase pausing by a riboswitch and its ligand.Nature structural & molecular biology · 2023Article
- Mechanism of transcription initiation and primer generation at the mitochondrial replication origin OriL.The EMBO journal · 2021Article
- The Mechanisms of Substrate Selection, Catalysis, and Translocation by the Elongating RNA Polymerase.Journal of molecular biology · 2019Review
- The cutting edge of archaeal transcription.Emerging topics in life sciences · 2018Article
- Non-programmed transcriptional frameshifting is common and highly RNA polymerase type-dependent.Microbial cell factories · 2018Article
- Structure and function of the N-terminal domain of the yeast telomerase reverse transcriptase.Nucleic acids research · 2018Article
- Visualizing translocation dynamics and nascent transcript errors in paused RNA polymerases in vivo.Genome biology · 2015Article
- Transcription elongation. Heterogeneous tracking of RNA polymerase and its biological implications.Transcription · 2014Review
- A dual switch controls bacterial enhancer-dependent transcription.Nucleic acids research · 2012Article
- Factor-independent transcription pausing caused by recognition of the RNA-DNA hybrid sequence.The EMBO journal · 2012Article
- Regulation of gene expression by reiterative transcription.Current opinion in microbiology · 2011Review
- The transcription inhibitor lipiarmycin blocks DNA fitting into the RNA polymerase catalytic site.The EMBO journal · 2010Article
- Deletion of switch 3 results in an archaeal RNA polymerase that is defective in transcript elongation.The Journal of biological chemistry · 2010Article
- Elongation by RNA polymerase: a race through roadblocks.Current opinion in structural biology · 2009Review
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
During transcription elongation the nascent RNA remains base-paired to the template strand of the DNA before it is displaced and the two strands of the DNA reanneal, resulting in the formation of a transcription "bubble" of approximately 10 bp. To examine how the length of the RNA-DNA hybrid is maintained, we assembled transcription elongation complexes on synthetic nucleic acid scaffolds that mimic the situation in which transcript displacement is compromised and the polymerase synthesizes an extended hybrid. We found that in such complexes bacterial RNA polymerase exhibit an intrinsic endonucleolytic cleavage activity that restores the hybrid to its normal length. Mutations in the region of the RNA polymerase near the site of RNA-DNA separation result in altered RNA displacement and translocation functions and as a consequence in different patterns of proofreading activities. Our data corroborate structural findings concerning the elements involved in the maintenance of the length of the RNA-DNA hybrid and suggest interplay between polymerase translocation, DNA strand separation, and intrinsic endonucleolytic activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.