ArticleMolecular microbiology2009
HlyU acts as an H-NS antirepressor in the regulation of the RTX toxin gene essential for the virulence of the human pathogen Vibrio vulnificus CMCP6.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Review
- Attenuation of a DNA cruciform by a conserved regulator directs T3SS1 mediated virulence in Vibrio parahaemolyticus.Nucleic acids research · 2023Article
- Increased intracellular persulfide levels attenuate HlyU-mediated hemolysin transcriptional activation inbioRxiv : the preprint server for biology · 2023Article
- Targeting Virulence Genes Expression inInternational journal of molecular sciences · 2022Article
- Bacterial Transcriptional Regulators: A Road Map for Functional, Structural, and Biophysical Characterization.International journal of molecular sciences · 2022Review
- Spatiotemporal Regulation ofToxins · 2020Review
- A MARTX ToxinmBio · 2020Article
- The transcriptional regulator IscR integrates host-derived nitrosative stress and iron starvation in activation of theThe Journal of biological chemistry · 2020Article
- The role of Vibrio vulnificus virulence factors and regulators in its infection-induced sepsis.Folia microbiologica · 2020Review
- Review
- Cold shock induces chromosomalAntimicrobial agents and chemotherapy · 2019Article
- The Transcriptional Regulator HlyU Positively Regulates Expression ofJournal of bacteriology · 2018Article
- Identification of 2',4'-Dihydroxychalcone as an Antivirulence Agent Targeting HlyU, a Master Virulence Regulator inMolecules (Basel, Switzerland) · 2018Article
- Article
- Article
- Identification and Validation of an Antivirulence Agent Targeting HlyU-Regulated Virulence inFrontiers in cellular and infection microbiology · 2018Article
- Expression of Hemolysin Is Regulated Under the Collective Actions of HapR, Fur, and HlyU inFrontiers in microbiology · 2018Article
- Identification and characterization ofThe Journal of biological chemistry · 2017Article
- H-NS: an overarching regulator of the Vibrio cholerae life cycle.Research in microbiology · 2017Review
- Phylogeny ofFrontiers in microbiology · 2017Article
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Authors and funding
3 authors.
Funding
Abstract
In Vibrio vulnificus, HlyU upregulates the expression of the large RTX toxin gene. In this work we identified the binding site of HlyU to -417 to -376 bp of the rtxA1 operon transcription start site. lacZ fusions for a series of progressive deletions from the rtxA1 operon promoter showed that transcriptional activity increased independently of HlyU when its binding site was absent. Thus HlyU must regulate the rtxA1 operon expression by antagonizing a negative regulator. Concomitantly we found that an hns mutant resulted in an increase in the expression of the rtxA1 operon genes. Multiple copies of HlyU can increase the promoter activity only in the presence of H-NS underscoring the hypothesis that HlyU must alleviate the repression by this protein. H-NS binds to a region that extends upstream and downstream of the rtxA1 operon promoter. In the upstream region it binds to five AT-rich sites of which two overlap the HlyU binding site. Competitive footprinting and gel shift data demonstrate HlyU's higher affinity as compared with H-NS resulting in the de-repression and a corresponding increased expression of the rtxA1 operon.
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