ArticleMolecular microbiology2009
Complex inheritance of the plasmodial surface anion channel in a Plasmodium falciparum genetic cross.
Article in Molecular microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Novel Ion Channel Genes in Malaria Parasites.Genes · 2024Review
- Epigenetics of malaria parasite nutrient uptake, but why?Trends in parasitology · 2022Review
- Non-ribosomal insights into ribosomal P2 protein in Plasmodium falciparum-infected erythrocytes.MicrobiologyOpen · 2021Article
- Complex nutrient channel phenotypes despite Mendelian inheritance in a Plasmodium falciparum genetic cross.PLoS pathogens · 2020Article
- Multiple genetic loci define CaBMC genomics · 2019Article
- The conserved clag multigene family of malaria parasites: essential roles in host-pathogen interaction.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2015Review
- A kinetic fluorescence assay reveals unusual features of Ca⁺⁺ uptake in Plasmodium falciparum-infected erythrocytes.Malaria journal · 2014Article
- Why do malaria parasites increase host erythrocyte permeability?Trends in parasitology · 2014Review
- High guanidinium permeability reveals dehydration-dependent ion selectivity in the plasmodial surface anion channel.BioMed research international · 2014Article
- Proteolysis at a specific extracellular residue implicates integral membrane CLAG3 in malaria parasite nutrient channels.PloS one · 2014Article
- Solute restriction reveals an essential role for clag3-associated channels in malaria parasite nutrient acquisition.Molecular pharmacology · 2012Article
- Ion and nutrient uptake by malaria parasite-infected erythrocytes.Cellular microbiology · 2012Review
- Genome scanning of Amazonian Plasmodium falciparum shows subtelomeric instability and clindamycin-resistant parasites.Genome research · 2010Article
- Altered plasmodial surface anion channel activity and in vitro resistance to permeating antimalarial compounds.Biochimica et biophysica acta · 2010Article
- Malaria parasite mutants with altered erythrocyte permeability: a new drug resistance mechanism and important molecular tool.Future microbiology · 2010Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Human erythrocytes infected with the malaria parasite Plasmodium falciparum have increased permeabilities to many solutes. The plasmodial surface anion channel (PSAC) may mediate these changes. Despite good understanding of the biochemical and biophysical properties, the genetic basis of PSAC activity remains unknown. Functional polymorphisms in laboratory isolates and two mutants generated by in vitro selection implicate a parasite-encoded channel, although parasite-induced modifications of endogenous channels have not been formally excluded. Here, we identified stable differences in furosemide efficacy against PSAC activity induced by HB3 and 3D7A parasites. This difference was apparent in both single PSAC patch-clamp recordings and in sorbitol-mediated osmotic lysis measurements, confirming that Cl(-) and sorbitol are transported by a single-channel type. Examination of 19 progeny from a genetic cross between HB3 and 3D7A revealed complex inheritance with some cloned progeny exhibiting furosemide affinities outside the range of parental values. Isolates generated by selfing of the 3D7A clone also exhibited altered furosemide affinities, implicating changes in one or more alleles during meiosis or passage through a primate host. PSAC may be encoded by multiple parasite genes (e.g. a multi-gene family or multiple genes that encode distinct channel subunits) or a single polymorphic gene under strong selective pressure.
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Registered trials
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