ArticleInternational journal of legal medicine2009
Suspension fluorescence in situ hybridization (S-FISH) combined with automatic detection and laser microdissection for STR profiling of male cells in male/female mixtures.
Article in International journal of legal medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 40 citations in OpenAlex.
- Evaluating novel and conventional cell-separation techniques for sexual assault investigations.Journal of forensic sciences · 2025Article
- Separation/extraction, detection, and interpretation of DNA mixtures in forensic science (review).International journal of legal medicine · 2018Review
- Isolating cells from female/male blood mixtures using florescence in situ hybridization combined with low volume PCR and its application in forensic science.International journal of legal medicine · 2015Article
- Y-chromosomal haplotyping of single sperm cells isolated from semen mixtures - a successful identification of three perpetrators in a multi-suspect sexual assault case.Croatian medical journal · 2014Article
- Beyond laser microdissection technology: follow the yellow brick road for cancer research.American journal of cancer research · 2014Review
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- Forensic trace DNA: a review.Investigative genetics · 2010Article
- Laser capture microdissection in forensic research: a review.International journal of legal medicine · 2010Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laser microdissection is a valuable tool for isolating specific cells from mixtures, such as male cells in a mixture with female cells, e.g., in cases of sexual assault. These cells can be stained with Y-chromosome-specific probes. We developed an automatic screening method to detect male cells after fluorescence in situ hybridization in suspension (S-FISH). To simulate forensic casework, the method was tested on female saliva after cataglottis (a kiss involving tongue-to-tongue contact) and on licking traces (swabs of dried male saliva on female skin) even after drying. After isolation of the detected cells, short tandem repeat profiling was performed. Full DNA profiles could consistently be obtained from as little as ten buccal cells. Isolation of five cells resulted in a mean of 98% (SD of 3.4%) of the alleles detected, showing that the developed S-FISH staining had no significant negative influence on DNA recovery and can be used in forensic casework.
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