ArticleJournal of translational medicine2009
MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells.
Article in Journal of translational medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 100 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
100 citing papers in PubMed, 182 citations in OpenAlex.
- NamiRNA-Enhancer Network: The Changing Era Challenge and Chance of miRNA.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Identification of RNA-based cell-type markers for stem-cell manufacturing systems with a statistical scoring function.Gene reports · 2024Article
- Review
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- Human Embryonic Stem-Cell-Derived Exosomes Repress NLRP3 Inflammasome to Alleviate Pyroptosis in Nucleus Pulposus Cells by Transmitting miR-302c.International journal of molecular sciences · 2023Article
- Wildtype heterogeneity contributes to clonal variability in genome edited cells.Scientific reports · 2022Article
- SHANK family on stem cell fate and development.Cell death & disease · 2022Review
- Role of PCK2 in the proliferation of vascular smooth muscle cells in neointimal hyperplasia.International journal of biological sciences · 2022Article
- Embryonal tumors with multilayered rosettes,Frontiers in oncology · 2022Article
- MicroRNA Expression Profile Analysis ofBioengineering (Basel, Switzerland) · 2021Article
- Inter-regulatory role of microRNAs in interaction between viruses and stem cells.World journal of stem cells · 2021Review
- Tissue-specific and transcription-dependent mechanisms regulate primary microRNA processing efficiency of the human chromosome 19 MicroRNA cluster.RNA biology · 2021Article
- MicroRNA-520c-3p suppresses vascular endothelium dysfunction by targeting RELA and regulating the AKT and NF-κB signaling pathways.Journal of physiology and biochemistry · 2021Article
- Effects of miR-125b-5p on Preadipocyte Proliferation and Differentiation in Chicken.Molecular biology reports · 2021Article
- miR-302d Competitively Binding with the lncRNA-341 Targets TLE4 in the Process of SSC Generation.Stem cells international · 2021Article
- Article
- miR-615 Fine-Tunes Growth and Development and Has a Role in Cancer and in Neural Repair.Cells · 2020Review
- MicroRNAomic Transcriptomic Analysis Reveal Deregulation of Clustered Cellular Functions in Human Mesenchymal Stem Cells During in Vitro Passaging.Stem cell reviews and reports · 2020Article
- Role of microRNA in inner ear stem cells and related research progress.American journal of stem cells · 2020Review
- Maternal obesity alters C19MC microRNAs expression profile in fetal umbilical cord blood.Nutrition & metabolism · 2020Article
40 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe unique features of human embryonic stem (hES) cells make them the best candidate resource for both cell replacement therapy and development research. However, the molecular mechanisms responsible for the simultaneous maintenance of their self-renewal properties and undifferentiated state remain unclear. Non-coding microRNAs (miRNA) which regulate mRNA cleavage and inhibit encoded protein translation exhibit temporal or tissue-specific expression patterns and they play an important role in development timing.
resultsIn this study, we analyzed miRNA and gene expression profiles among samples from 3 hES cell lines (H9, I6 and BG01v), differentiated embryoid bodies (EB) derived from H9 cells at different time points, and 5 adult cell types including Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal Human Astrocytes (NHA), and Lung Fibroblasts (LFB). This analysis rendered 104 miRNAs and 776 genes differentially expressed among the three cell types. Selected differentially expressed miRNAs and genes were further validated and confirmed by quantitative real-time-PCR (qRT-PCR). Especially, members of the miR-302 cluster on chromosome 4 and miR-520 cluster on chromosome 19 were highly expressed in undifferentiated hES cells. MiRNAs in these two clusters displayed similar expression levels. The members of these two clusters share a consensus 7-mer seed sequence and their targeted genes had overlapping functions. Among the targeted genes, genes with chromatin structure modification function are enriched suggesting a role in the maintenance of chromatin structure. We also found that the expression level of members of the two clusters, miR-520b and miR-302c, were negatively correlated with their targeted genes based on gene expression analysis
conclusionWe identified the expression patterns of miRNAs and gene transcripts in the undifferentiation of human embryonic stem cells; among the miRNAs that are highly expressed in undifferentiated embryonic stem cells, the miR-520 cluster may be closely involved in hES cell function and its relevance to chromatin structure warrants further study.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.