ArticlePlant cell reports2009
Evidence for stable transformation of wheat by floral dip in Agrobacterium tumefaciens.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 123 citations in OpenAlex.
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- Multi-modal few-shot learning for anthesis prediction of individual wheat plants.Plant phenomics (Washington, D.C.) · 2025Article
- Agrobacterium-Mediated Genetic Transformation in Wheat.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Review
- A Rapid Method for Obtaining the Transgenic Roots of Crassulaceae Plants.Plants (Basel, Switzerland) · 2024Article
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- Efficient floral dip transformation method usingSaudi journal of biological sciences · 2023Article
- CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops.Frontiers in plant science · 2023Review
- An efficient transformation method for genome editing of elite bread wheat cultivars.Frontiers in plant science · 2023Article
- Germline transformation ofBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Development and optimization of a Barley stripe mosaic virus-mediated gene editing system to improve Fusarium head blight resistance in wheat.Plant biotechnology journal · 2022Article
- Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience.Plants (Basel, Switzerland) · 2021Review
- Genetic Transformation of Apomictic Grasses: Progress and Constraints.Frontiers in plant science · 2021Review
- The present and potential future methods for delivering CRISPR/Cas9 components in plants.Journal, genetic engineering & biotechnology · 2020Review
- Article
- Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID).BMC research notes · 2019Article
- Genetic Modification for Wheat Improvement: From Transgenesis to Genome Editing.BioMed research international · 2019Review
- Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat.Scientific reports · 2018Article
- An in planta biolistic method for stable wheat transformation.Scientific reports · 2017Article
- A Tightly Regulated Genetic Selection System with Signaling-Active Alleles of Phytochrome B.Plant physiology · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hexaploid wheat, one of the world's most important staple crops, remains a challenge for genetic transformation. We are developing a floral transformation protocol for wheat that does not require tissue culture. This paper presents three transformants in the hard red germplasm line Crocus that have been characterized thoroughly at the molecular level over three to six generations. Wheat spikes at the early boot stage, i.e. the early, mid or late uninucleate microspore stages, were immersed in an infiltration medium of strain C58C1 harboring pDs(Hyg)35S, or strain AGL1 harboring pBECKSred. pDs(Hyg)35S contains the NPTII and hph selectable markers, and transformants were detected using paromomycin spray at the whole plant level, NPTII ELISAs, or selection on medium with hygromycin. Strain AGL1, harboring pBECKSred, which contains the maize anthocyanin regulators, Lc and C1, and the NPTII gene, was also used to produce a Crocus transformant. T1 and T2 seeds with red embryos were selected; T1 and T2 plants were screened by sequential tests for paromomycin resistance and NPTII ELISAs. The transformants were low copy number and showed Mendelian segregation in the T2. Stable transmission of the transgenes over several generations has been demonstrated using Southern analysis. Gene expression in advanced progeny was shown using Reverse Transcriptase-PCR and ELISA assays for NPTII protein expression. This protocol has the potential to reduce the time and expense required for wheat transformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.