ArticleMolecular and cellular biology2009
Differential cofactor requirements for histone eviction from two nucleosomes at the yeast PHO84 promoter are determined by intrinsic nucleosome stability.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins inACS synthetic biology · 2024Article
- Optogenetic control of phosphate-responsive genes using single component fusion proteins inbioRxiv : the preprint server for biology · 2024Article
- Nucleosome Remodeling at the YeastInternational journal of molecular sciences · 2023Article
- The Active Mechanism of Nucleosome Depletion by Poly(dA:dT) Tracts In Vivo.International journal of molecular sciences · 2021Review
- Article
- Rice nucleosome patterns undergo remodeling coincident with stress-induced gene expression.BMC genomics · 2018Article
- Two Distinct Regulatory Mechanisms of Transcriptional Initiation in Response to Nutrient Signaling.Genetics · 2018Article
- Emerging Trends in Epigenetic Regulation of Nutrient Deficiency Response in Plants.Molecular biotechnology · 2016Article
- Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast.Molecular and cellular biology · 2015Article
- Mediator, TATA-binding protein, and RNA polymerase II contribute to low histone occupancy at active gene promoters in yeast.The Journal of biological chemistry · 2014Article
- The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening.Nucleic acids research · 2014Article
- The yeast PHO5 promoter: from single locus to systems biology of a paradigm for gene regulation through chromatin.Nucleic acids research · 2014Review
- Bimodal expression of PHO84 is modulated by early termination of antisense transcription.Nature structural & molecular biology · 2013Article
- Article
- Phosphate disruption and metal toxicity in Saccharomyces cerevisiae: effects of RAD23 and the histone chaperone HPC2.Biochemical and biophysical research communications · 2012Article
- Chromatin and transcription in yeast.Genetics · 2012Review
- Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe.Nucleic acids research · 2011Article
- The RSC chromatin remodelling enzyme has a unique role in directing the accurate positioning of nucleosomes.The EMBO journal · 2011Article
- The INO80 ATP-dependent chromatin remodeling complex is a nucleosome spacing factor.Molecular and cellular biology · 2011Article
- Quantitative trait loci, epigenetics, sugars, and microRNAs: quaternaries in phosphate acquisition and use.Plant physiology · 2010Article
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We showed previously that the strong PHO5 promoter is less dependent on chromatin cofactors than the weaker coregulated PHO8 promoter. In this study we asked if chromatin remodeling at the even stronger PHO84 promoter was correspondingly less cofactor dependent. The repressed PHO84 promoter showed a short hypersensitive region that was flanked upstream and downstream by a positioned nucleosome and contained two transactivator Pho4 sites. Promoter induction generated an extensive hypersensitive and histone-depleted region, yielding two more Pho4 sites accessible. This remodeling was strictly Pho4 dependent, strongly dependent on the remodelers Snf2 and Ino80 and on the histone acetyltransferase Gcn5, and more weakly on the acetyltransferase Rtt109. Importantly, remodeling of each of the two positioned nucleosomes required Snf2 and Ino80 to different degrees. Only remodeling of the upstream nucleosome was strictly dependent on Snf2. Further, remodeling of the upstream nucleosome was more dependent on Ino80 than remodeling of the downstream nucleosome. Both nucleosomes differed in their intrinsic stabilities as predicted in silico and measured in vitro. The causal relationship between the different nucleosome stabilities and the different cofactor requirements was shown by introducing destabilizing mutations in vivo. Therefore, chromatin cofactor requirements were determined by intrinsic nucleosome stabilities rather than correlated to promoter strength.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.