ArticleThe Journal of biological chemistry2009
Mutants of the Arabidopsis thaliana cation/H+ antiporter AtNHX1 conferring increased salt tolerance in yeast: the endosome/prevacuolar compartment is a target for salt toxicity.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 81 citations in OpenAlex.
- Genome-wide identification and gene expression analysis of AhNHXs in peanut.BMC plant biology · 2026Article
- Structural and Functional Characterization of the Vacuolar-Type NaBiomolecules · 2025Article
- Structure-Guided Identification of Critical Residues in the Vacuolar Cation/Proton Antiporter NHX1 fromPlants (Basel, Switzerland) · 2023Article
- Gain-of-function mutations of AtNHX1 suppress sos1 salt sensitivity and improve salt tolerance in Arabidopsis.Stress biology · 2021Article
- New Insights into the Structure-Function Relationship of the Endosomal-Type NaInternational journal of molecular sciences · 2020Article
- Coordinated Transport of Nitrate, Potassium, and Sodium.Frontiers in plant science · 2020Review
- Expression of AoNHX1 increases salt tolerance of rice and Arabidopsis, and bHLH transcription factors regulate AtNHX1 and AtNHX6 in Arabidopsis.Plant cell reports · 2019Article
- Mutual Regulation of Receptor-Like Kinase SIT1 and B'κ-PP2A Shapes the Early Response of Rice to Salt Stress.The Plant cell · 2019Article
- The lichen symbiosis re-viewed through the genomes of Cladonia grayi and its algal partner Asterochloris glomerata.BMC genomics · 2019Article
- The Effect of AtHKT1;1 or AtSOS1 Mutation on the Expressions of Na⁺ or K⁺ Transporter Genes and Ion Homeostasis inInternational journal of molecular sciences · 2019Article
- Antarctic Extremophiles: Biotechnological Alternative to Crop Productivity in Saline Soils.Frontiers in bioengineering and biotechnology · 2019Article
- KPlant physiology · 2018Article
- Plant Endomembrane Dynamics: Studies of KPlant physiology · 2018Review
- Expression and integrated network analyses revealed functional divergence of NHX-type NaScientific reports · 2017Article
- Vacuolar H(+)-Pyrophosphatase AVP1 is Involved in Amine Fungicide Tolerance in Arabidopsis thaliana and Provides Tridemorph Resistance in Yeast.Frontiers in plant science · 2016Article
- Root-endophytes improve the ecophysiological performance and production of an agricultural species under drought condition.AoB PLANTS · 2016Article
- Overexpression of a Vesicle Trafficking Gene, OsRab7, enhances salt tolerance in rice.TheScientificWorldJournal · 2014Article
- Different evolutionary histories of two cation/proton exchanger gene families in plants.BMC plant biology · 2013Article
- Physiological and molecular mechanisms of plant salt tolerance.Photosynthesis research · 2013Review
- Functional characterization of a wheat NHX antiporter gene TaNHX2 that encodes a K(+)/H(+) exchanger.PloS one · 2013Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mutants of the plant cation/H(+) antiporter AtNHX1 that confer greater halotolerance were generated by random mutagenesis and selected in yeast by phenotypic complementation. The amino acid substitutions that were selected were conservative and occurred in the second half of the membrane-associated N terminus. AtNHX1 complemented the lack of endogenous ScNHX1 in endosomal protein trafficking assays. Growth enhancement on hygromycin B and vanadate media agreed with a generally improved endosomal/prevacuolar function of the mutated proteins. In vivo measurements by (31)P NMR revealed that wild-type and mutant AtNHX1 transporters did not affect cytosolic or vacuolar pH. Surprisingly, when yeast cells were challenged with lithium, a tracer for sodium, the main effect of the mutations in AtNHX1 was a reduction in the amount of compartmentalized lithium. When purified and reconstituted into proteoliposomes or assayed in intact vacuoles isolated from yeast cells, a representative mutant transporter (V318I) showed a greater cation discrimination favoring potassium transport over that of sodium or lithium. Together, our data suggest that the endosome/prevacuolar compartment is a target for salt toxicity. Poisoning by toxic cations in the endosome/prevacuolar compartment is detrimental for cell functions, but it can be alleviated by improving the discrimination of transported alkali cations by the resident cation/H(+) antiporter.
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