ArticlePlanta2009
CYP710A genes encoding sterol C22-desaturase in Physcomitrella patens as molecular evidence for the evolutionary conservation of a sterol biosynthetic pathway in plants.
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Stigmasterol: An Enigmatic Plant Stress Sterol with Versatile Functions.International journal of molecular sciences · 2024Review
- Identification of P450 Candidates Associated with the Biosynthesis of Physalin-Class Compounds inInternational journal of molecular sciences · 2023Article
- Biosynthesis and the Roles of Plant Sterols in Development and Stress Responses.International journal of molecular sciences · 2022Review
- Phytohormone biosynthesis and signaling pathways of mosses.Plant molecular biology · 2021Review
- Structural and functional analysis of tomato sterol C22 desaturase.BMC plant biology · 2021Article
- Changes in the Plant β-Sitosterol/Stigmasterol Ratio Caused by the Plant Parasitic NematodePlants (Basel, Switzerland) · 2021Article
- Phytosterol Profiles, Genomes and Enzymes - An Overview.Frontiers in plant science · 2021Review
- MaizeFrontiers in plant science · 2021Article
- Membrane Lipids, Waxes and Oxylipins in the Moss Model Organism Physcomitrella patens.Plant & cell physiology · 2019Review
- Why Do Plants Convert Sitosterol to Stigmasterol?Frontiers in plant science · 2019Review
- Triterpene Structural Diversification by Plant Cytochrome P450 Enzymes.Frontiers in plant science · 2017Review
- Plant cytochrome P450s: nomenclature and involvement in natural product biosynthesis.Protoplasma · 2016Review
- Fungal cytochrome p450 monooxygenases: their distribution, structure, functions, family expansion, and evolutionary origin.Genome biology and evolution · 2014Article
- Metabolic engineering of the moss Physcomitrella patens to produce the sesquiterpenoids patchoulol and α/β-santalene.Frontiers in plant science · 2014Article
- Plant P450s as versatile drivers for evolution of species-specific chemical diversity.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Biosynthesis of cholesterol and other sterols.Chemical reviews · 2011Review
- Cytochromes p450.The arabidopsis book · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have characterized cytochromes P450, CYP710A13, and CYP710A14, as the sterol C22-desaturase in the moss Physcomitrella patens. GC-MS analyses demonstrated that P. patens accumulated stigmasterol as the major sterol (56-60% of total sterol) and sitosterol to a lesser extent (8-12%); this sterol profile contrasts with those in higher plants accumulating stigmasterol as a minor component. Recombinant CYP710A13 and CYP710A14 proteins prepared using a baculovirus/insect cell system exhibited the C22-desaturase activity with beta-sitosterol to produce stigmasterol, while campesterol and 24-epi-campesterol were not accepted as the substrates. The K(m) values for beta-sitosterol of CYP710A13 (1.0 +/- 0.043 microM) and CYP710A14 (2.1 +/- 0.17 microM) were at comparable levels of those reported with higher plant CYP710A proteins. In Arabidopsis T87 cells over-expressing CYP710A14, stigmasterol contents reached a level 20- to 72-fold higher than those in the basal level of T87 cells, confirming the C22-desaturase activity of this P450 enzyme. The occurrence of the end-products together with the enzymes involved in the last step of the pathway substantiated the presence of an entire sterol biosynthetic pathway in P. patens, providing evidence for the conservation of the sterol biosynthetic pathway through the evolutionary process of land plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.