ArticlePlanta2009
Expression of a multi-epitope DPT fusion protein in transplastomic tobacco plants retains both antigenicity and immunogenicity of all three components of the functional oligomer.
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Unleashing the immune arsenal: development of broad spectrum multiepitope bluetongue vaccine targeting conserved T cell epitopes of structural proteins.BMC genomics · 2026Article
- Hosts and Heterologous Expression Strategies of Recombinant Toxins for Therapeutic Purposes.Toxins · 2023Review
- Overexpression of the recombinant human interferon-beta (Biotechnologia · 2021Article
- Antibodies induced by oral immunization of mice with a recombinant protein produced in tobacco plants harboringPlant cell, tissue and organ culture · 2021Article
- An AMA1/MSP1Molecular biotechnology · 2020Article
- Optimization of rPDT fusion protein expression by Escherichia coli in pilot scale fermentation: a statistical experimental design approach.AMB Express · 2018Article
- Chloroplast genomes: diversity, evolution, and applications in genetic engineering.Genome biology · 2016Review
- Expression of a Codon-Optimized dsdA Gene in Tobacco Plastids and Rice Nucleus Confers D-Serine Tolerance.Frontiers in plant science · 2016Article
- Chloroplast localization of Cry1Ac and Cry2A protein--an alternative way of insect control in cotton.Biological research · 2015Article
- Expression of the Multimeric and Highly Immunogenic Brucella spp. Lumazine Synthase Fused to Bovine Rotavirus VP8d as a Scaffold for Antigen Production in Tobacco Chloroplasts.Frontiers in plant science · 2015Article
- Expression of the truncated tissue plasminogen activator (K2S) gene in tobacco chloroplast.Molecular biology reports · 2013Article
- Article
- Plastid genetic engineering in Solanaceae.Protoplasma · 2012Review
- Article
- Two decades of plant-based candidate vaccines: a review of the chimeric protein approaches.Plant cell reports · 2011Review
- Oral immunogenicity of tomato-derived sDPT polypeptide containing Corynebacterium diphtheriae, Bordetella pertussis and Clostridium tetani exotoxin epitopes.Plant cell reports · 2011Article
- Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons.Plant cell reports · 2010Article
- Tobacco, a highly efficient green bioreactor for production of therapeutic proteins.Biotechnology advancesReview
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of genes in plant chloroplasts provides an opportunity for enhanced production of target proteins. We report the introduction and expression of a fusion DPT protein containing immunoprotective exotoxin epitopes of Corynebacterium diphtheriae, Bordetella pertussis, and Clostridium tetani in tobacco chloroplasts. Using biolistic-mediated transformation, a plant-optimized synthetic DPT gene was successfully transferred to tobacco plastomes. Putative transplastomic T0 plants were identified by PCR, and Southern blot analysis confirmed homoplasmy in T1 progeny. ELISA assays demonstrated that the DPT protein retained antigenicity of the three components of the fusion protein. The highest level of expression in these transplastomic plants reached 0.8% of total soluble protein. To assess whether the functional recombinant protein expressed in tobacco plants would induce specific antibodies in test animals, a mice feeding experiment was conducted. For mice orally immunized with freeze-dried transplastomic leaves, production of IgG and IgA antibodies specific to each toxin were detected in serum and mucosal tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.