ArticleNucleic acids research2009
A conserved 3' extension in unusual group II introns is important for efficient second-step splicing.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Group II Introns: Flexibility and Repurposing.Frontiers in molecular biosciences · 2022Article
- Evolution of group II introns.Mobile DNA · 2015Article
- Novel RNA structural features of an alternatively splicing group II intron from Clostridium tetani.RNA (New York, N.Y.) · 2014Article
- Diversity, mobility, and structural and functional evolution of group II introns carrying an unusual 3' extension.BMC research notes · 2011Article
- Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4.Environmental microbiology · 2011Article
- Group II introns: mobile ribozymes that invade DNA.Cold Spring Harbor perspectives in biology · 2011Review
- Linking the branchpoint helix to a newly found receptor allows lariat formation by a group II intron.The EMBO journal · 2011Article
- The tertiary structure of group II introns: implications for biological function and evolution.Critical reviews in biochemistry and molecular biology · 2010Review
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Authors and funding
3 authors.
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Abstract
The B.c.I4 group II intron from Bacillus cereus ATCC 10987 harbors an unusual 3' extension. Here, we report the discovery of four additional group II introns with a similar 3' extension in Bacillus thuringiensis kurstaki 4D1 that splice at analogous positions 53/56 nt downstream of domain VI in vivo. Phylogenetic analyses revealed that the introns are only 47-61% identical to each other. Strikingly, they do not form a single evolutionary lineage even though they belong to the same Bacterial B class. The extension of these introns is predicted to form a conserved two-stem-loop structure. Mutational analysis in vitro showed that the smaller stem S1 is not critical for self-splicing, whereas the larger stem S2 is important for efficient exon ligation and lariat release in presence of the extension. This study clearly demonstrates that previously reported B.c.I4 is not a single example of a specialized intron, but forms a new functional class with an unusual mode that ensures proper positioning of the 3' splice site.
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