ArticleRNA (New York, N.Y.)2009
Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.
Article in RNA (New York, N.Y.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- Domain acquisition enabled functional expansion of the TFIIS transcription factor family.Cell & bioscience · 2025Review
- The regulation and function of post-transcriptional RNA splicing.Nature reviews. Genetics · 2025Review
- Birth of a poly(A) tail: mechanisms and control of mRNA polyadenylation.FEBS open bio · 2023Review
- Slight Variations in the Sequence Downstream of the Polyadenylation Signal Significantly Increase Transgene Expression in HEK293T and CHO Cells.International journal of molecular sciences · 2022Article
- Nuclear and cytoplasmic poly(A) binding proteins (PABPs) favor distinct transcripts and isoforms.Nucleic acids research · 2022Article
- Nuclear mRNA Quality Control and Cytoplasmic NMD Are Linked by the Guard Proteins Gbp2 and Hrb1.International journal of molecular sciences · 2021Review
- Functional impacts of non-coding RNA processing on enhancer activity and target gene expression.Journal of molecular cell biology · 2019Review
- Long ncRNA A-ROD activates its target gene DKK1 at its release from chromatin.Nature communications · 2018Article
- Integration of mRNP formation and export.Cellular and molecular life sciences : CMLS · 2017Review
- Temporal Dissection of Rate Limiting Transcriptional Events Using Pol II ChIP and RNA Analysis of Adrenergic Stress Gene Activation.PloS one · 2015Article
- Article
- TRAP150 activates splicing in composite terminal exons.Nucleic acids research · 2014Article
- Article
- Competition within Introns: Splicing Wins over Polyadenylation via a General Mechanism.Acta naturae · 2013Article
- Splicing kinetics and transcript release from the chromatin compartment limit the rate of Lipid A-induced gene expression.RNA (New York, N.Y.) · 2013Article
- U1snRNP-mediated suppression of polyadenylation in conjunction with the RNA structure controls poly (A) site selection in foamy viruses.Retrovirology · 2013Article
- Autoregulation of TDP-43 mRNA levels involves interplay between transcription, splicing, and alternative polyA site selection.Genes & development · 2012Article
- Polyuridylylation and processing of transcripts from multiple gene minicircles in chloroplasts of the dinoflagellate Amphidinium carterae.Plant molecular biology · 2012Article
- Article
- Modelling reveals kinetic advantages of co-transcriptional splicing.PLoS computational biology · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
When transcription is coupled to pre-mRNA processing in HeLa nuclear extracts nascent transcripts become attached to RNA polymerase II during assembly of the cleavage/polyadenylation apparatus (CPA), and are not released even after cleavage at the poly(A) site. Here we show that these cleaved transcripts are anchored to the polymerase at their 3' ends by the CPA or, when introns are present, by the larger 3'-terminal exon definition complex (EDC), which consists of splicing factors complexed with the CPA. Poly(A) addition releases the RNA from the polymerase when the RNA is anchored only by the CPA. When anchored by the EDC, poly(A) addition remains a requirement, but it triggers release only after being licensed by splicing. The process by which RNA must first be attached to the polymerase by the EDC, and then can only be released following dual inputs from splicing and polyadenylation, provides an obvious opportunity for surveillance as the RNA enters the transport pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.