ArticleAmerican journal of physiology. Lung cellular and molecular physiology2009
Paracrine purinergic signaling determines lung endothelial nitric oxide production.
Article in American journal of physiology. Lung cellular and molecular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Lung Single-Cell Transcriptomics Reveal Diverging Pathobiology and Opportunities for Precision Targeting in Scleroderma-Associated Versus Idiopathic Pulmonary Arterial Hypertension.Circulation. Genomic and precision medicine · 2025Article
- Purinergic regulation of pulmonary vascular tone.Purinergic signalling · 2024Review
- Studying the Pulmonary Endothelium in Health and Disease: An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology · 2024Article
- Release of ATP in the lung evoked by inhalation of irritant gases in rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Article
- Rescue of alveolar wall liquid secretion blocks fatal lung injury due to influenza-staphylococcal coinfection.The Journal of clinical investigation · 2023Article
- Endothelial dysfunction triggers acute respiratory distress syndrome in patients with sepsis: a narrative review.Frontiers in medicine · 2023Review
- The P2-receptor-mediated CaPurinergic signalling · 2019Article
- Application of a novel in vivo imaging approach to measure pulmonary vascular responses in mice.Physiological reports · 2018Article
- Disruption of staphylococcal aggregation protects against lethal lung injury.The Journal of clinical investigation · 2018Article
- Mathematical model for shear stress dependent NO and adenine nucleotide production from endothelial cells.Nitric oxide : biology and chemistry · 2016Article
- Novel regulators of endothelial barrier function.American journal of physiology. Lung cellular and molecular physiology · 2014Review
- F-actin scaffold stabilizes lamellar bodies during surfactant secretion.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Live imaging of the lung.Annual review of physiology · 2014Review
- Lipopolysaccharide induces endoplasmic store Ca2+-dependent inflammatory responses in lung microvessels.PloS one · 2013Article
- Endothelial connexin43 mediates acid-induced increases in pulmonary microvascular permeability.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- Spontaneous lung dysfunction and fibrosis in mice lacking connexin 40 and endothelial cell connexin 43.The American journal of pathology · 2011Article
- Activation of TNFR1 ectodomain shedding by mitochondrial Ca2+ determines the severity of inflammation in mouse lung microvessels.The Journal of clinical investigation · 2011Article
- P2Y1 and P2Y13 purinergic receptors mediate Ca2+ signaling and proliferative responses in pulmonary artery vasa vasorum endothelial cells.American journal of physiology. Cell physiology · 2011Article
- The role of purinergic signaling on deformation induced injury and repair responses of alveolar epithelial cells.PloS one · 2011Article
- Human alveolar type II cells secrete and absorb liquid in response to local nucleotide signaling.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Although the vascular bed is a major source of nitric oxide (NO) production, factors regulating the production remain unclear. We considered the role played by paracrine signaling. Determinations by fluorescence microscopy in isolated, blood-perfused rat and mouse lungs revealed that a brief lung expansion enhanced cytosolic Ca(2+) (Ca(2+)cyt) oscillations in alveolar epithelial (AEC) and endothelial (EC) cells, and NO production in EC. Furthermore, as assessed by a novel microlavage assay, alveolar ATP production increased. Intra-alveolar microinfusion of the purinergic receptor antagonist, PPADS, and the nucleotide hydrolyzing enzyme, apyrase, each completely blocked the Ca(2+)cyt and NO responses in EC. Lung expansion induced Ca(2+)cyt oscillations in mice lacking the P2Y1, but not the P2Y2, purinergic receptors, which were located in the perivascular interstitium basolateral to AEC. Prolonged lung expansion instituted by mechanical ventilation at high tidal volume increased EC expression of nitrotyrosine, indicating development of nitrosative stress in lung microvessels. These findings reveal a novel mechanism in which mechanically induced purinergic signaling couples cross-compartmental Ca(2+)cyt oscillations to microvascular NO production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.