ArticleJournal of bacteriology2009
Sialic acid (N-acetyl neuraminic acid) utilization by Bacteroides fragilis requires a novel N-acetyl mannosamine epimerase.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 80 citations in OpenAlex.
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- Expanding salivary biomarker detection by creating a synthetic neuraminic acid sensor via chimeragenesis.Applied and environmental microbiology · 2026Article
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- Mucin Type Drives Composition and Mucin Glycan Degradation of an In Vitro Synthetic Microbial Community.Environmental microbiology · 2026Article
- Sialidases and sialylated oligosaccharides in intestinal homeostasis and inflammation.Frontiers in immunology · 2026Review
- Structural Basis for Tetramerization ofJournal of microbiology and biotechnology · 2025Article
- Structure-dependent degradation of milk oligosaccharides by newly isolated intestinal commensal bacterial strains from suckling piglets and rabbits.BMC microbiology · 2025Article
- The cervical microbiome of ewe breeds with known divergent fertility following artificial insemination with frozen-thawed semen.Scientific reports · 2025Article
- A comparative study of the substrate preference of the sialidases, CpNanI, HpNanH, and BbSia2 towards 2-Aminobenzamide-labeled 3'-Sialyllactose, 6'-Sialyllactose, and Sialyllacto-N-tetraose-b.Biochemistry and biophysics reports · 2024Article
- Nutrient acquisition strategies by gut microbes.Cell host & microbe · 2024Review
- A bacterial sialidase mediates early-life colonization by a pioneering gut commensal.Cell host & microbe · 2024Article
- Starvation responses impact interaction dynamics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis.The ISME journal · 2023Article
- Bacterial Sialic Acid Catabolism at the Host-Microbe Interface.Journal of microbiology (Seoul, Korea) · 2023Review
- A bioorthogonal chemistry approach to detect the K1 polysialic acid capsule inRSC chemical biology · 2023Article
- Comparative Genomics Revealed a Potential Threat of Aeromonas rivipollensis G87 Strain and Its Antibiotic ResistanceAntibiotics (Basel, Switzerland) · 2023Article
- Metagenomic survey reveals global distribution and evolution of microbial sialic acid catabolism.Frontiers in microbiology · 2023Article
- A Blood-Based Metabolite Panel for Distinguishing Ovarian Cancer from Benign Pelvic Masses.Clinical cancer research : an official journal of the American Association for Cancer Research · 2022Article
- Enhancement of Edwardsiella piscicida infection, biofilm formation, and motility caused by N-acetylneuraminate lyase.Glycoconjugate journal · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
We characterized the nanLET operon in Bacteroides fragilis, whose products are required for the utilization of the sialic acid N-acetyl neuraminic acid (NANA) as a carbon and energy source. The first gene of the operon is nanL, which codes for an aldolase that cleaves NANA into N-acetyl mannosamine (manNAc) and pyruvate. The next gene, nanE, codes for a manNAc/N-acetylglucosamine (NAG) epimerase, which, intriguingly, possesses more similarity to eukaryotic renin binding proteins than to other bacterial NanE epimerase proteins. Unphosphorylated manNAc is the substrate of NanE, while ATP is a cofactor in the epimerase reaction. The third gene of the operon is nanT, which shows similarity to the major transporter facilitator superfamily and is most likely to be a NANA transporter. Deletion of any of these genes eliminates the ability of B. fragilis to grow on NANA. Although B. fragilis does not normally grow with manNAc as the sole carbon source, we isolated a B. fragilis mutant strain that can grow on this substrate, likely due to a mutation in a NAG transporter; both manNAc transport and NAG transport are affected in this strain. Deletion of the nanE epimerase gene or the rokA hexokinase gene, whose product phosphorylates NAG, in the manNAc-enabled strain abolishes growth on manNAc. Thus, B. fragilis possesses a new pathway of NANA utilization, which we show is also found in other Bacteroides species.
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