ArticleJournal of bacteriology2009
The biosynthetic pathway for myxol-2' fucoside (myxoxanthophyll) in the cyanobacterium Synechococcus sp. strain PCC 7002.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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36 citing papers in PubMed, 82 citations in OpenAlex.
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- Proximity Labeling Facilitates Defining the Proteome Neighborhood of Photosystem II Oxygen Evolution Complex in a Model Cyanobacterium.Molecular & cellular proteomics : MCP · 2022Article
- Carotenoids and Their Biosynthesis in Fungi.Molecules (Basel, Switzerland) · 2022Review
- Xanthophylls from the Sea: Algae as Source of Bioactive Carotenoids.Marine drugs · 2021Review
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- Diversity and Evolution of Carotenoid Biosynthesis from Prokaryotes to Plants.Advances in experimental medicine and biology · 2021Review
- An Overview on Industrial and Medical Applications of Bio-Pigments Synthesized by Marine Bacteria.Microorganisms · 2020Review
- Current knowledge and recent advances in understanding metabolism of the model cyanobacterium Synechocystis sp. PCC 6803.Bioscience reports · 2020Review
- Deciphering Pathways for Carotenogenesis in Haloarchaea.Molecules (Basel, Switzerland) · 2020Article
- Synthesis, Regulation and Degradation of Carotenoids Under Low Level UV-B Radiation in the Filamentous CyanobacteriumFrontiers in microbiology · 2020Article
- Proteome Mapping of a Cyanobacterium Reveals Distinct Compartment Organization and Cell-Dispersed Metabolism.Plant physiology · 2019Article
- Biosynthetic Potential of a Novel Antarctic ActinobacteriumMarine drugs · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synechococcus sp. strain PCC 7002 produces a variety of carotenoids, which comprise predominantly dicylic beta-carotene and two dicyclic xanthophylls, zeaxanthin and synechoxanthin. However, this cyanobacterium also produces a monocyclic myxoxanthophyll, which was identified as myxol-2' fucoside. Compared to the carotenoid glycosides produced by diverse microorganisms, cyanobacterial myxoxanthophyll and closely related compounds are unusual because they are glycosylated on the 2'-OH rather than on the 1'-OH position of the psi end of the molecule. In this study, the genes encoding two enzymes that modify the psi end of myxoxanthophyll in Synechococcus sp. strain PCC 7002 were identified. Mutational and biochemical studies showed that open reading frame SynPCC7002_A2032, renamed cruF, encodes a 1',2'-hydroxylase [corrected] and that open reading frame SynPCC7002_A2031, renamed cruG, encodes a 2'-O-glycosyltransferase. The enzymatic activity of CruF was verified by chemical characterization of the carotenoid products synthesized when cruF was expressed in a lycopene-producing strain of Escherichia coli. Database searches showed that homologs of cruF and cruG occur in the genomes of all sequenced cyanobacterial strains that are known to produce myxol or the acylic xanthophyll oscillaxanthin. The genomes of many other bacteria that produce hydroxylated carotenoids but do not contain crtC homologs also contain cruF orthologs. Based upon observable intermediates, a complete biosynthetic pathway for myxoxanthophyll is proposed. This study expands the suite of enzymes available for metabolic engineering of carotenoid biosynthetic pathways for biotechnological applications.
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