ArticleApplied and environmental microbiology2009
Development of cpn60-based real-time quantitative PCR assays for the detection of 14 Campylobacter species and application to screening of canine fecal samples.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 84 citations in OpenAlex.
- Trial
- Enteropathy produced in mice by intergenerational transmission of small intestinal microbiota from undernourished children.Nature microbiology · 2026Article
- Effects of intergenerational transmission of small intestinal bacteria cultured from stunted Bangladeshi children with enteropathy.bioRxiv : the preprint server for biology · 2025Article
- Determinants of Campylobacter species diversity in infants and association with family members, livestock, and household environments in rural Eastern Ethiopia.Gut pathogens · 2025Article
- "My Bitch Is Empty!" An Overview of the Reasons for Pregnancy Loss in Dogs.Veterinary sciences · 2025Review
- Article
- Potentially zoonotic pathogens and parasites in opportunistically sourced urban brown rats (Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Prevalence and Load of the Campylobacter Genus in Infants and Associated Household Contacts in Rural Eastern Ethiopia: a Longitudinal Study from the Campylobacter Genomics and Environmental Enteric Dysfunction (CAGED) Project.Applied and environmental microbiology · 2023Article
- Rapid and accurate taxonomic classification of cpn60 amplicon sequence variants.ISME communications · 2023Article
- Foodborne Pathogens at the Livestock-Wildlife-Human Interface in Rural Western Uganda.EcoHealth · 2023Article
- Unravelling the reservoirs for colonisation of infants withBMJ open · 2022Article
- A Case of Persistent Diarrhea in a Man with the Molecular Detection of VariousPathogens (Basel, Switzerland) · 2020Article
- Whole genome sequencing reveals extended natural transformation in Campylobacter impacting diagnostics and the pathogens adaptive potential.Scientific reports · 2020Article
- Campylobacter culture fails to correctly detect Campylobacter in 30% of positive patient stool specimens compared to non-cultural methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- Prevalence and risk factors associated with Campylobacter spp. occurrence in healthy dogs visiting four rural community veterinary clinics in South Africa.The Onderstepoort journal of veterinary research · 2019Article
- Update on cpnDB: a reference database of chaperonin sequences.Database : the journal of biological databases and curation · 2019Article
- A New Highly Sensitive and Specific Real-Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens.Journal of clinical microbiology · 2018Article
- Antimicrobial resistantGut pathogens · 2018Article
- Evaluation of long-acting oxytetracycline and a commercial monovalent vaccine for the control ofThe Canadian veterinary journal = La revue veterinaire canadienne · 2017Article
- Incidence ofGut pathogens · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter species are important organisms in both human and animal health. The identification of Campylobacter currently requires the growth of organisms from complex samples and biochemical identification. In many cases, the condition of the sample being tested and/or the fastidious nature of many Campylobacter species has limited the detection of campylobacters in a laboratory setting. To address this, we have designed a set of real-time quantitative PCR (qPCR) assays to detect and quantify 14 Campylobacter species, C. coli, C. concisus, C. curvus, C. fetus, C. gracilis, C. helveticus, C. hyointestinalis, C. jejuni, C. lari, C. mucosalis, C. rectus, C. showae, C. sputorum, and C. upsaliensis, directly from DNA extracted from feces. By use of a region of the cpn60 (also known as hsp60 or groEL) gene, which encodes the universally conserved 60-kDa chaperonin, species-specific assays were designed and validated. These assays were then employed to determine the prevalence of Campylobacter species in fecal samples from dogs. Fecal samples were found to contain detectable and quantifiable levels of C. fetus, C. gracilis, C. helveticus, C. jejuni, C. showae, and C. upsaliensis, with the majority of samples containing multiple Campylobacter species. This study represents the first report of C. fetus, C. gracilis, C. mucosalis, and C. showae detection in dogs and implicates dogs as a reservoir for these species. The qPCR assays described offer investigators a new tool to study many Campylobacter species in a culture-independent manner.
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