ArticleApplied and environmental microbiology2009
Failure to detect Helicobacter pylori DNA in drinking and environmental water in Dhaka, Bangladesh, using highly sensitive real-time PCR assays.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- On the Role of Food in the Transmission ofFoods (Basel, Switzerland) · 2025Review
- Effect of dilution factor on the isolation ofIranian journal of microbiology · 2022Article
- Efficacy of Loop-Mediated Isothermal Amplification forDiagnostics (Basel, Switzerland) · 2021Article
- The Puzzle of Coccoid Forms ofAntibiotics (Basel, Switzerland) · 2020Review
- Potential transmission sources ofJournal of environmental health science & engineering · 2019Article
- Review
- Microbial Interaction as a Determinant of the Quality of Supply Drinking Water: A Conceptual Analysis.Frontiers in public health · 2018Article
- CD4Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association · 2017Article
- Helicobacter pylori isolated from Iranian drinking water: vacA, cagA, iceA, oipA and babA2 genotype status and antimicrobial resistance properties.FEBS open bio · 2016Article
- Identification of a Latin American-specific BabA adhesin variant through whole genome sequencing of Helicobacter pylori patient isolates from Nicaragua.BMC evolutionary biology · 2016Article
- Contaminated water as a source of Helicobacter pylori infection: A review.Journal of advanced research · 2015Review
- Three Tests Used to Identify Non-Culturable Form of Helicobacter pylori in Water Samples.Jundishapur journal of microbiology · 2015Article
- Methods for Detecting the Environmental Coccoid Form of Helicobacter pylori.Frontiers in public health · 2015Review
- Temporal dynamics in the free-living bacterial community composition in the coastal North Sea.FEMS microbiology ecology · 2013Article
- Detection of pathogens in water: from phylochips to qPCR to pyrosequencing.Current opinion in biotechnology · 2012Review
- Optimizing the growth of stressed Helicobacter pylori.Journal of microbiological methods · 2011Article
- Article
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Authors and funding
6 authors.
Funding
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Abstract
The main transmission pathway of Helicobacter pylori has not been determined, but several reports have described detection of H. pylori DNA in drinking and environmental water, suggesting that H. pylori may be waterborne. To address this possibility, we developed, tested, and optimized two complementary H. pylori-specific real-time PCR assays for quantification of H. pylori DNA in water. The minimum detection level of the assays including collection procedures and DNA extraction was shown to be approximately 250 H. pylori genomes per water sample. Using our assays, we then analyzed samples of drinking and environmental water (n = 75) and natural water biofilms (n = 21) from a high-endemicity area in Bangladesh. We could not identify H. pylori DNA in any of the samples, even though other pathogenic bacteria have been found previously in the same water samples by using the same methodology. A series of control experiments were performed to ensure that the negative results were not falsely caused by PCR inhibition, nonspecific assays, degradation of template DNA, or low detection sensitivity. Our results suggest that it is unlikely that the predominant transmission route of H. pylori in this area is waterborne.
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