ArticleExperimental parasitology2009
Leishmania (Viannia) panamensis: an in vitro assay using the expression of GFP for screening of antileishmanial drug.
Article in Experimental parasitology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Colombian Contributions Fighting Leishmaniasis: A Systematic Review on Antileishmanials Combined with Chemoinformatics Analysis.Molecules (Basel, Switzerland) · 2020Pooled it
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- Antileishmanial activity of a new chloroquine analog in an animal model of Leishmania panamensis infection.International journal for parasitology. Drugs and drug resistance · 2020Article
- Intraspecies differences in natural susceptibility to amphotericine B of clinical isolates of Leishmania subgenus Viannia.PloS one · 2018Article
- Mitochondria and lipid raft-located FOF1-ATP synthase as major therapeutic targets in the antileishmanial and anticancer activities of ether lipid edelfosine.PLoS neglected tropical diseases · 2017Article
- Local Delivery of the Toll-Like Receptor 9 Ligand CpG Downregulates Host Immune and Inflammatory Responses, Ameliorating Established Leishmania (Viannia) panamensis Chronic Infection.Infection and immunity · 2017Article
- Novel cell-based in vitro screen to identify small-molecule inhibitors against intracellular replication of Cryptococcus neoformans in macrophages.International journal of antimicrobial agents · 2016Article
- Development of a novel formulation with hypericin to treat cutaneous leishmaniasis based on photodynamic therapy in in vitro and in vivo studies.Antimicrobial agents and chemotherapy · 2015Article
- Antileishmanial Effect of 5,3'-Hydroxy-7,4'-dimethoxyflavanone of Picramnia gracilis Tul. (Picramniaceae) Fruit: In Vitro and In Vivo Studies.Advances in pharmacological sciences · 2015Article
- A Review: The Current In Vivo Models for the Discovery and Utility of New Anti-leishmanial Drugs Targeting Cutaneous Leishmaniasis.PLoS neglected tropical diseases · 2015Review
- Leishmaniasis Panamensis Masquerading as Myiasis and Sporotrichosis: A Clinical Pitfall.Case reports in pathology · 2015Article
- The immunotherapeutic role of regulatory T cells in Leishmania (Viannia) panamensis infection.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Downregulation of Calcineurin Gene Is Associated with Glucantime(®) Resiatance in Leishmania infantum.Iranian journal of parasitology · 2013Article
- An alternative in vitro drug screening test using Leishmania amazonensis transfected with red fluorescent protein.Diagnostic microbiology and infectious disease · 2013Article
- In vitro and in vivo efficacy of ether lipid edelfosine against Leishmania spp. and SbV-resistant parasites.PLoS neglected tropical diseases · 2012Article
- Use of in vivo and in vitro systems to select Leishmania amazonensis expressing green fluorescent protein.The Korean journal of parasitology · 2011Article
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- Flow cytometric screening for anti-leishmanials in a human macrophage cell line.Experimental parasitology · 2010Article
- Selective fusion of azurophilic granules with Leishmania-containing phagosomes in human neutrophils.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Promastigotes of Leishmania (Viannia) panamensis were successfully transfected with p6.5-egfp to express green fluorescent protein. The transfectants remained infective to macrophages, providing an in vitro model for screening antileishmanial drugs. This was demonstrated by flow cytometry of macrophage-associated GFP after exposure of infected cultures to known antileishmanial drugs, i.e. amphotericin B and glucantime. Fluorescence of GFP diminished progressively from infected cells with increasing drug concentrations used in both cases. The availability of this fluorescent assay for infection of macrophages by L. (V.) panamensis facilitates drug discovery program for the Viannia species, which differ significantly from those of the Leishmania subgenus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.