ArticleAging cell2009
Autophagy and amino acid homeostasis are required for chronological longevity in Saccharomyces cerevisiae.
Article in Aging cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 137 papers.
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Who cites it
137 citing papers in PubMed, 226 citations in OpenAlex.
- The hallmarks of protein and amino acid restriction in aging and longevity.Cell press blue · 2026Article
- The YeastInternational journal of molecular sciences · 2026Article
- Sir2 regulates selective autophagy in stationary-phase yeast cells.Microbial cell (Graz, Austria) · 2026Article
- Metabolic Biomarkers for Lifespan Extension and Cellular Stress inPreventive nutrition and food science · 2025Article
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- The core genetic drivers of chronological aging in yeast are universal regulators of longevity.Microbial cell (Graz, Austria) · 2025Article
- Multi-Omics Analysis Reveals the Toxicity of Polyvinyl Chloride Microplastics toward BEAS-2B Cells.Toxics · 2024Article
- Induction of proteasomal activity in mammalian cells by lifespan-extending tRNA synthetase inhibitors.GeroScience · 2024Article
- Cardiovascular aging: the mitochondrial influence.The journal of cardiovascular aging · 2023Article
- An evaluation of aging measures: from biomarkers to clocks.Biogerontology · 2023Review
- Saccharomyces cerevisiae responds similarly to co-culture or to a fraction enriched in Metschnikowia pulcherrima extracellular vesicles.Microbial biotechnology · 2023Article
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- Review
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- Lifespan benefits for the combination of rapamycin plus acarbose and for captopril in genetically heterogeneous mice.Aging cell · 2022Article
- Quiescence inAnnual review of genetics · 2022Review
- Rewiring of the protein-protein-metabolite interactome during the diauxic shift in yeast.Cellular and molecular life sciences : CMLS · 2022Article
- NUMB facilitates autophagy initiation through targeting SCFCell death and differentiation · 2022Article
- Review
- Proteomic and metabolomic analysis of Nicotiana benthamiana under dark stress.FEBS open bio · 2022Article
77 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Following cessation of growth, yeast cells remain viable in a nondividing state for a period of time known as the chronological lifespan (CLS). Autophagy is a degradative process responsible for amino acid recycling in response to nitrogen starvation and amino acid limitation. We have investigated the role of autophagy during chronological aging of yeast grown in glucose minimal media containing different supplemental essential and nonessential amino acids. Deletion of ATG1 or ATG7, both of which are required for autophagy, reduced CLS, whereas deletion of ATG11, which is required for selective targeting of cellular components to the vacuole for degradation, did not reduce CLS. The nonessential amino acids isoleucine and valine, and the essential amino acid leucine, extended CLS in autophagy-deficient as well as autophagy-competent yeast. This extension was suppressed by constitutive expression of GCN4, which encodes a transcriptional regulator of general amino acid control (GAAC). Consistent with this, GCN4 expression was reduced by isoleucine and valine. Furthermore, elimination of the leucine requirement extended CLS and prevented the effects of constitutive expression of GCN4. Interestingly, deletion of LEU3, a GAAC target gene encoding a transcriptional regulator of branched side chain amino acid synthesis, dramatically increased CLS in the absence of amino acid supplements. In general, this indicates that activation of GAAC reduces CLS whereas suppression of GAAC extends CLS in minimal medium. These findings demonstrate important roles for autophagy and amino acid homeostasis in determining CLS in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.