ArticleClinical and experimental immunology2009
Complement classical pathway components are all important in clearance of apoptotic and secondary necrotic cells.
Article in Clinical and experimental immunology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 106 citations in OpenAlex.
- Exploring the causal effect of complement and IgA nephropathy-a Mendelian randomization study.Renal failure · 2024Article
- Hepatocyte nuclear factor 4α is a critical factor for the production of complement components in the liver.In vitro cellular & developmental biology. Animal · 2024Article
- Complement activation drives the phagocytosis of necrotic cell debris and resolution of liver injury.Frontiers in immunology · 2024Article
- Increased infiltration of CD4BMC immunology · 2023Article
- Microglia complement signaling promotes neuronal elimination and normal brain functional connectivity.Cerebral cortex (New York, N.Y. : 1991) · 2023Article
- Immunopathology of terminal complement activation and complement C5 blockade creating a pro-survival and organ-protective phenotype in trauma.British journal of pharmacology · 2023Article
- The Factor H protein family: The switchers of the complement alternative pathway.Immunological reviews · 2023Review
- Molecular signatures of intrarenal complement receptorsLupus science & medicine · 2022Article
- Complement 4 Aids in the Prediction of Newly Diagnosed Multiple Myeloma Outcome in Patients.Clinical Medicine Insights. Oncology · 2022Article
- Complement Receptors and Their Role in Leukocyte Recruitment and Phagocytosis.Frontiers in cell and developmental biology · 2021Review
- Complement deposition, C4d, on platelets is associated with vascular events in systemic lupus erythematosus.Rheumatology (Oxford, England) · 2020Article
- Phagocytosis of Necrotic Debris at Sites of Injury and Inflammation.Frontiers in immunology · 2019Review
- Extracellular DNA and autoimmune diseases.Cellular & molecular immunology · 2018Review
- Article
- Pyroptosis triggers pore-induced intracellular traps (PITs) that capture bacteria and lead to their clearance by efferocytosis.The Journal of experimental medicine · 2016Article
- The dual role of complement in cancer and its implication in anti-tumor therapy.Annals of translational medicine · 2016Review
- Complement in Lupus Nephritis: New Perspectives.Kidney diseases (Basel, Switzerland) · 2015Review
- Alternative complement pathway component Factor D contributes to efficient clearance of tissue debris following acute CCl₄-induced injury.Molecular immunology · 2015Article
- Complement deficiency promotes cutaneous wound healing in mice.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Complement C4 induces regulatory T cells differentiation through dendritic cell in systemic lupus erythematosus.Cell & bioscience · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inherited deficiencies in components of the classical complement pathway are strong disease susceptibility factors for the development of systemic lupus erythematosus (SLE) and there is a hierarchy among deficiency states, the strongest association being with C1q deficiency. We investigated the relative importance of the different complement pathways regarding clearance of apoptotic cells. Phagocytosis of labelled apoptotic Jurkat cells by monocyte-derived macrophages in the presence of sera from individuals with complement deficiencies was studied, as well as C3 deposition on apoptotic cells using flow cytometry. Sera from individuals deficient in C1q, C4, C2 or C3 all showed decreased phagocytosis. Mannose binding lectin (MBL) and the alternative pathway did not influence phagocytosis. Notably, the components of the complement classical pathway, including C1q, were equally important in clearance of apoptotic cells. This indicates that deposition of C3 fragments is of major significance; we therefore studied C3 deposition on apoptotic cells. Experiments with MBL-deficient serum depleted of C1q or factor D confirmed the predominance of the classical pathway. At low dilution, sera deficient of C1q, C4 or C2 supported C3 fragment deposition demonstrating alternative pathway activation. In conclusion, we have found that complement-mediated opsonization and phagocytosis of apoptotic cells, particularly those undergoing secondary necrosis, are dependent mainly upon an intact classical pathway. The alternative pathway is less important, but may play a role in some conditions. C1q was not more important than other classical pathway components, suggesting a role in additional pathogenetic processes in SLE other than clearance of apoptotic cells.
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