ArticleArchives of virology2009
Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model.
Article in Archives of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 75 citations in OpenAlex.
- Development of an HRM-qRT-PCR platform for fast and cost-effective genotyping of infectious bronchitis virus in Egypt.Scientific reports · 2026Article
- Australian strains of the avian coronavirus infectious bronchitis virus predominantly target the respiratory tract rather than the kidneys in specific-pathogen-free chickens.The Journal of general virology · 2026Article
- Isolation, molecular identification, and phylogenetic analysis of infectious bronchitis virus from commercial chicken farms in Mekele and Bishoftu, Ethiopia, 2023-2024.Virology journal · 2025Article
- Avian infectious bronchitis virus (AIBV) review by continent.Frontiers in cellular and infection microbiology · 2024Review
- Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.PloS one · 2021Article
- Molecular Characterization of Infectious Bronchitis Virus Strain HH06 Isolated in a Poultry Farm in Northeastern China.Frontiers in veterinary science · 2021Article
- Pathological effect of infectious bronchitis disease virus on broiler chicken trachea and kidney tissues.Veterinary world · 2020Article
- Characterization of Sicilian rosemary (Rosmarinus officinalis L.) germplasm through a multidisciplinary approach.Planta · 2020Article
- Molecular characterization of a unique variant of avian infectious bronchitis virus in Tunisia.Poultry science · 2019Article
- Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I.The Journal of veterinary medical science · 2018Article
- Identification and molecular characterization of a novel serotype infectious bronchitis virus (GI-28) in China.Veterinary microbiology · 2017Article
- Productive replication of nephropathogenic infectious bronchitis virus in peripheral blood monocytic cells, a strategy for viral dissemination and kidney infection in chickens.Veterinary research · 2016Article
- Pathogenesis and Diagnostic Approaches of Avian Infectious Bronchitis.Advances in virology · 2016Review
- Analysis of the complete genomic sequences of two virus subpopulations of the Australian infectious bronchitis virus vaccine VicS.Avian pathology : journal of the W.V.P.A · 2015Article
- DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece.Molecular biology reports · 2014Article
- Assessment of the potential relationship between egg quality and infectious bronchitis virus infection in Australian layer flocks.Australian veterinary journal · 2014Article
- Molecular detection of infectious bronchitis and Newcastle disease viruses in broiler chickens with respiratory signs using Duplex RT-PCR.Veterinary research forum : an international quarterly journal · 2014Article
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- High-resolution melting analysis as a powerful tool to discriminate and genotype Pseudomonas savastanoi pathovars and strains.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infectious bronchitis virus (IBV) is a coronavirus that causes upper respiratory, renal and/or reproductive diseases with high morbidity in poultry. Classification of IBV is important for implementation of vaccination strategies to control the disease in commercial poultry. Currently, the lengthy process of sequence analysis of the IBV S1 gene is considered the gold standard for IBV strain identification, with a high nucleotide identity (e.g. > or =95%) indicating related strains. However, this gene has a high propensity to mutate and/or undergo recombination, and alone it may not be reliable for strain identification. A real-time polymerase chain reaction (RT-PCR) combined with high-resolution melt (HRM) curve analysis was developed based on the 3'UTR of IBV for rapid detection and classification of IBV from commercial poultry. HRM curves generated from 230 to 435-bp PCR products of several IBV strains were subjected to further analysis using a mathematical model also developed during this study. It was shown that a combination of HRM curve analysis and the mathematical model could reliably group 189 out of 190 comparisons of pairs of IBV strains in accordance with their 3'UTR and S1 gene identities. The newly developed RT-PCR/HRM curve analysis model could detect and rapidly identify novel and vaccine-related IBV strains, as confirmed by S1 gene and 3'UTR nucleotide sequences. This model is a rapid, reliable, accurate and non-subjective system for detection of IBVs in poultry flocks.
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