ReviewExtremophiles : life under extreme conditions2009
Applications of flow cytometry in environmental microbiology and biotechnology.
Review in Extremophiles : life under extreme conditions, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- The influence of environmental factors on the diversity and structure of airborne fungal and bacterial communities.Mycology · 2026Review
- A comprehensive review of microbial contamination in the indoor environment: sources, sampling, health risks, and mitigation strategies.Frontiers in public health · 2023Review
- Development of a flow cytometry-based plating-free system for strain engineering in industrial fungi.Applied microbiology and biotechnology · 2022Article
- Initial evenness determines diversity and cell density dynamics in synthetic microbial ecosystems.Scientific reports · 2018Article
- Flow Cytometry Sorting to Separate Viable Giant Viruses from Amoeba Co-culture Supernatants.Frontiers in cellular and infection microbiology · 2016Article
- Assessment of total bacterial cells in extended aeration activated sludge plants using flow cytometry as a microbial monitoring tool.Environmental science and pollution research international · 2015Article
- Attachment and detachment of living microorganisms using a potential-controlled electrode.Marine biotechnology (New York, N.Y.) · 2013Article
- A method for detecting forward scattering signals on-chip with a photonic-microfluidic integrated device.Biomedical optics express · 2013Article
- Impact of asymptomatic Plasmodium falciparum parasitemia on the imunohematological indices among school children and adolescents in a rural area highly endemic for malaria in southern Mozambique.BMC infectious diseases · 2013Article
- The future is now: single-cell genomics of bacteria and archaea.FEMS microbiology reviews · 2013Review
- Cell sorting enriches Escherichia coli mutants that rely on peptidoglycan endopeptidases to suppress highly aberrant morphologies.Journal of bacteriology · 2013Article
- Directed molecular evolution to design advanced red fluorescent proteins.Nature methods · 2011Article
- Applications of flow cytometry in plant pathology for genome size determination, detection and physiological status.Molecular plant pathology · 2011Review
- Morphological plasticity promotes resistance to phagocyte killing of uropathogenic Escherichia coli.Microbes and infection · 2011Article
- Engineering cytochrome P450 biocatalysts for biotechnology, medicine and bioremediation.Expert opinion on drug metabolism & toxicology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedure known as random or neutral drift where screening and selection is based on the retention of some predetermined level of activity through multiple rounds of mutagenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.