ArticleJournal of cell science2009
Stability of the small gamma-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus.
Article in Journal of cell science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Combining bioinformatics and machine learning to analyze and validate sepsis-related cell senescence genes and potential drugs.Renal failure · 2026Article
- Signaling to make human ribosomes: Connections between the cytoplasm and the nucleolus.Molecular cell · 2026Review
- Validating RRP12 Expression and Its Prognostic Significance in HCC Based on Data Mining and Bioinformatics Methods.Frontiers in oncology · 2022Article
- Cyanidin 3-glucoside modulated cell cycle progression in liver precancerous lesion,World journal of gastroenterology · 2021Article
- Neurymenolide A, a Novel Mitotic Spindle Poison from the New Caledonian RhodophytaMarine drugs · 2019Article
- Regulation of microtubule nucleation mediated by γ-tubulin complexes.Protoplasma · 2017Review
- Emerging genotype-phenotype relationships in patients with large NF1 deletions.Human genetics · 2017Review
- Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth.Cellular and molecular life sciences : CMLS · 2015Article
- Centrosomal nucleolin is required for microtubule network organization.Cell cycle (Georgetown, Tex.) · 2015Article
- hCLE/C14orf166 associates with DDX1-HSPC117-FAM98B in a novel transcription-dependent shuttling RNA-transporting complex.PloS one · 2014Article
- Par6γ is at the mother centriole and controls centrosomal protein composition through a Par6α-dependent pathway.Journal of cell science · 2013Article
- Mammalian HCA66 protein is required for both ribosome synthesis and centriole duplication.Nucleic acids research · 2012Article
- Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response.Molecular and cellular biology · 2011Article
- Double duty for nuclear proteins--the price of more open forms of mitosis.Trends in genetics : TIG · 2009Review
- Arabidopsis cortical microtubules are initiated along, as well as branching from, existing microtubules.The Plant cell · 2009Article
- On the origin of cortical microtubules.The Plant cell · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
To investigate changes at the centrosome during the cell cycle, we analyzed the composition of the pericentriolar material from unsynchronized and S-phase-arrested cells by gel electrophoresis and mass spectrometry. We identified HCA66, a protein that localizes to the centrosome from S-phase to mitosis and to the nucleolus throughout interphase. Silencing of HCA66 expression resulted in failure of centrosome duplication and in the formation of monopolar spindles, reminiscent of the phenotype observed after gamma-tubulin silencing. Immunofluorescence microscopy showed that proteins of the gamma-tubulin ring complex were absent from the centrosome in these monopolar spindles. Immunoblotting revealed reduced protein levels of all components of the gamma-tubulin small complex (gamma-tubulin, GCP2, and GCP3) in HCA66-depleted cells. By contrast, the levels of gamma-tubulin ring complex proteins such as GCP4 and GCP-WD/NEDD1 were unaffected. We propose that HCA66 is a novel regulator of gamma-tubulin function that plays a role in stabilizing components of the gamma-tubulin small complex, which is in turn essential for assembling the larger gamma-tubulin ring complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.