Evidence map›Paper›PMID 19299317›Full record

ArticleBiology of reproduction2009

Maturation, fertilization, and the structure and function of the endoplasmic reticulum in cryopreserved mouse oocytes.

Katie M Lowther, Vanessa N Weitzman, Donald Maier, Lisa M Mehlmann

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Trial
  3. Trial
  4. Article
  5. Article
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  7. Article
  8. Article
  9. Cryopreservation of Gametes and Embryos and Their Molecular Changes.International journal of molecular sciences · 2021
    Review
  10. Article
  11. Proteomic Changes of Porcine Oocytes After Vitrification and SubsequentFrontiers in cell and developmental biology · 2020
    Article
  12. Article
  13. Article
  14. Recent progress in cryopreservation of bovine oocytes.BioMed research international · 2014
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Katie M LowtherDepartment of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
Vanessa N Weitzman
Donald Maier
Lisa M Mehlmann

Funding

Signaling mechanisms that regulate meiosis in mammalian oocytesR01HD056366 · NICHD · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI MEHLMANN, LISA M · 2007 to 2011
$1.3M
Cytoplasmic Maturation in Mouse OocytesR56HD056366 · NICHD · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI MEHLMANN, LISA M · 2017 to 2017
$300k
NICHD NIH HHS HD056366NICHD NIH HHS R01 HD056366NICHD NIH HHS R56 HD056366
6 · The paper itself

Abstract

Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction. Most studies examining the effects of cryopreservation on fertilization and developmental competence have been done using metaphase II-stage oocytes, while fewer studies have focused on freezing oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation. Herein, we examined the effects of vitrifying GV-stage mouse oocytes on cytoplasmic structure and on the ability to undergo cytoplasmic changes necessary for proper fertilization and early embryonic development. We examined the endoplasmic reticulum (ER) as one indicator of cytoplasmic structure, as well as the ability of oocytes to develop Ca(2+) release mechanisms following vitrification and in vitro maturation. Vitrified GV-stage oocytes matured in culture to metaphase II at a rate comparable to that of controls. These oocytes had the capacity to release Ca(2+) following injection of inositol 1,4,5-trisphosphate, demonstrating that Ca(2+) release mechanisms developed during meiotic maturation. The ER remained intact during the vitrification procedure as assessed using the lipophilic fluorescent dye DiI. However, the reorganization of the ER that occurs during in vivo maturation was impaired in oocytes that were vitrified before oocyte maturation. These results show that vitrification of GV-stage oocytes does not affect nuclear maturation or the continuity of the ER, but normal cytoplasmic maturation as assessed by the reorganization of the ER is disrupted. Deficiencies in factors that are responsible for proper ER reorganization during oocyte maturation could contribute to the low developmental potential previously reported in vitrified in vitro-matured oocytes.

Indexed as

CryopreservationOocytesAnimalsCell Culture TechniquesCells, CulturedEndoplasmic ReticulumFemaleFertilizationFertilization in VitroFreezingMeiosisMiceOogenesis

Identifiers

PMID19299317
PMCPMC3093990

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.