ArticleJournal of immunological methods2009
Ex vivo expansion of memory CD8 T cells from lymph nodes or spleen through in vitro culture with interleukin-7.
Article in Journal of immunological methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- The major isoforms of Bim contribute to distinct biological activities that govern the processes of autophagy and apoptosis in interleukin-7 dependent lymphocytes.Biochimica et biophysica acta · 2012Article
- JunD/AP-1-mediated gene expression promotes lymphocyte growth dependent on interleukin-7 signal transduction.PloS one · 2012Article
- Ex vivo expansion of tumor-reactive T cells by means of bryostatin 1/ionomycin and the common gamma chain cytokines formulation.Journal of visualized experiments : JoVE · 2011Article
- Cdc25A-driven proliferation regulates CD62L levels and lymphocyte movement in response to interleukin-7.Experimental hematology · 2010Article
- Interleukin-7 enhances memory CD8(+) T-cell recall responses in health but its activity is impaired in human immunodeficiency virus infection.Immunology · 2010Article
- The interaction of LCK and the CD4 co-receptor alters the dose response of T-cells to interleukin-7.Immunology letters · 2010Article
- Interleukin-7 mediates glucose utilization in lymphocytes through transcriptional regulation of the hexokinase II gene.American journal of physiology. Cell physiology · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
Interleukin-7 (IL-7) increases lymphocyte numbers, a critical feature of immune reconstitution, through mechanisms that are still poorly understood. Part of the problem is that IL-7 is produced in limited amounts by non-lymphoid cells, making in vivo studies of the cytokine's activity a challenge. To overcome this, we developed an in vitro system by which lymphocytes from secondary immune organs could be cultured to produce IL-7 responsive cells. Using this method, we showed that CD8(hi)CD44(hi) T cells accumulate in culture with IL-7 from a population of lymph node or splenic cells. These results were validated when a similar lymphocyte subset was found in mice expressing a constitutively active form of STAT5b, a key transducer of IL-7 signals. Interestingly, IL-7-expanded cells also up regulated the activation marker, CD69. The IL-7-derived CD44(hi)CD69(hi) cells were not generated from naïve cells, but expanded from an existing population, since culture in IL-7 of naïve lymphocytes from OT-1/Rag1(-/-) mice did not produce CD44(hi)CD69(hi) cells. Using the in vitro culture system to study lymphocytes from mice deficient in the apoptotic protein, BIM, we were able to attribute the expansion of CD8(hi)CD44(hi)CD69(hi) T cells to the proliferative and not survival activity of IL-7. The in vitro culture system provides an important new methodology to examine the activities of this essential as well as immunotherapeutic cytokine.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.