ArticleExperimental cell research2009
Dynamic analysis of apoptosis using cyanine SYTO probes: from classical to microfluidic cytometry.
Article in Experimental cell research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation.Methods and protocols · 2022Article
- Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe.Nucleic acids research · 2018Article
- Acoustofluidic chemical waveform generator and switch.Analytical chemistry · 2014Article
- The microfluidic multitrap nanophysiometer for hematologic cancer cell characterization reveals temporal sensitivity of the calcein-AM efflux assay.Scientific reports · 2014Article
- Multivariate analysis of apoptotic markers versus cell cycle phase in living human cancer cells by microfluidic cytometry.Proceedings of SPIE--the International Society for Optical Engineering · 2013Article
- Tunable, pulsatile chemical gradient generation via acoustically driven oscillating bubbles.Lab on a chip · 2013Article
- Real-time cell viability assays using a new anthracycline derivative DRAQ7®.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Article
- Cytometry of apoptosis. Historical perspective and new advances.Experimental oncology · 2012Review
- Rapid image-based cytometry for comparison of fluorescent viability staining methods.Journal of fluorescence · 2012Article
- Apoptosis goes on a chip: advances in the microfluidic analysis of programmed cell death.Analytical chemistry · 2011Article
- Analysis of individual molecular events of DNA damage response by flow- and image-assisted cytometry.Methods in cell biology · 2011Review
- Rise of the micromachines: microfluidics and the future of cytometry.Methods in cell biology · 2011Article
- Real-time cytotoxicity assays.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Rapid quantification of cell viability and apoptosis in B-cell lymphoma cultures using cyanine SYTO probes.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Cytometry in cell necrobiology revisited. Recent advances and new vistas.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Review
- Rationale for the real-time and dynamic cell death assays using propidium iodide.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2010Article
- Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells.Analytical chemistry · 2009Article
- Microfluidic single-cell array cytometry for the analysis of tumor apoptosis.Analytical chemistry · 2009Article
- DNA damage signaling assessed in individual cells in relation to the cell cycle phase and induction of apoptosis.Critical reviews in clinical laboratory sciencesReview
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Cell death is a stochastic process, often initiated and/or executed in a multi-pathway/multi-organelle fashion. Therefore, high-throughput single-cell analysis platforms are required to provide detailed characterization of kinetics and mechanisms of cell death in heterogeneous cell populations. However, there is still a largely unmet need for inert fluorescent probes, suitable for prolonged kinetic studies. Here, we compare the use of innovative adaptation of unsymmetrical SYTO dyes for dynamic real-time analysis of apoptosis in conventional as well as microfluidic chip-based systems. We show that cyanine SYTO probes allow non-invasive tracking of intracellular events over extended time. Easy handling and "stain-no wash" protocols open up new opportunities for high-throughput analysis and live-cell sorting. Furthermore, SYTO probes are easily adaptable for detection of cell death using automated microfluidic chip-based cytometry. Overall, the combined use of SYTO probes and state-of-the-art Lab-on-a-Chip platform emerges as a cost effective solution for automated drug screening compared to conventional Annexin V or TUNEL assays. In particular, it should allow for dynamic analysis of samples where low cell number has so far been an obstacle, e.g. primary cancer stems cells or circulating minimal residual tumors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.