ArticleMolecular biology of the cell2009
Prm3p is a pheromone-induced peripheral nuclear envelope protein required for yeast nuclear fusion.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- Nuclear Fusion in Yeast and Plant Reproduction.Plants (Basel, Switzerland) · 2023Article
- Effectors of the spindle assembly checkpoint are confined within the nucleus of Saccharomyces cerevisiae.Biology open · 2019Article
- Comparative genomics of nuclear envelope proteins.BMC genomics · 2018Article
- Article
- Growth condition dependency is the major cause of non-responsiveness upon genetic perturbation.PloS one · 2017Article
- The Malleable Nature of the Budding Yeast Nuclear Envelope: Flares, Fusion, and Fenestrations.Journal of cellular physiology · 2016Review
- Article
- Heterotrimeric G Protein-coupled Receptor Signaling in Yeast Mating Pheromone Response.The Journal of biological chemistry · 2016Review
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Kar5p is required for multiple functions in both inner and outer nuclear envelope fusion in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2014Article
- ER-associated SNAREs and Sey1p mediate nuclear fusion at two distinct steps during yeast mating.Molecular biology of the cell · 2013Article
- New Ca(2+)-dependent regulators of autophagosome maturation.Communicative & integrative biology · 2012Article
- An unusual transmembrane helix in the endoplasmic reticulum ubiquitin ligase Doa10 modulates degradation of its cognate E2 enzyme.The Journal of biological chemistry · 2011Article
- The final conformation of the complete ectodomain of the HA2 subunit of influenza hemagglutinin can by itself drive low pH-dependent fusion.The Journal of biological chemistry · 2011Article
- Article
- Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1.The Journal of biological chemistry · 2010Article
- Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.The Journal of biological chemistry · 2009Article
- Distinct roles for key karyogamy proteins during yeast nuclear fusion.Molecular biology of the cell · 2009Article
- Cytosol-dependent membrane fusion in ER, nuclear envelope and nuclear pore assembly: biological implications.Nucleus (Austin, Tex.)Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Nuclear membrane fusion is the last step in the mating pathway of the yeast Saccharomyces cerevisiae. We adapted a bioinformatics approach to identify putative pheromone-induced membrane proteins potentially required for nuclear membrane fusion. One protein, Prm3p, was found to be required for nuclear membrane fusion; disruption of PRM3 caused a strong bilateral defect, in which nuclear congression was completed but fusion did not occur. Prm3p was localized to the nuclear envelope in pheromone-responding cells, with significant colocalization with the spindle pole body in zygotes. A previous report, using a truncated protein, claimed that Prm3p is localized to the inner nuclear envelope. Based on biochemistry, immunoelectron microscopy and live cell microscopy, we find that functional Prm3p is a peripheral membrane protein exposed on the cytoplasmic face of the outer nuclear envelope. In support of this, mutations in a putative nuclear localization sequence had no effect on full-length protein function or localization. In contrast, point mutations and deletions in the highly conserved hydrophobic carboxy-terminal domain disrupted both protein function and localization. Genetic analysis, colocalization, and biochemical experiments indicate that Prm3p interacts directly with Kar5p, suggesting that nuclear membrane fusion is mediated by a protein complex.
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