ArticleDevelopment (Cambridge, England)2009
RacGAP50C directs perinuclear gamma-tubulin localization to organize the uniform microtubule array required for Drosophila myotube extension.
Article in Development (Cambridge, England), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- Myotube Guidance: Shaping up the Musculoskeletal System.Journal of developmental biology · 2024Review
- The serine/threonine kinase Back seat driver prevents cell fusion to maintain cell identity.Developmental biology · 2023Article
- Spatiotemporal expression of regulatory kinases directs the transition from mitosis to cellular morphogenesis in Drosophila.Nature communications · 2022Article
- A genetic screen for regulators of muscle morphogenesis in Drosophila.G3 (Bethesda, Md.) · 2021Article
- An insight on Drosophila myogenesis and its assessment techniques.Molecular biology reports · 2020Review
- Review
- FGF signaling directs myotube guidance by regulating Rac activity.Development (Cambridge, England) · 2020Article
- TheThe Journal of cell biology · 2019Article
- The Neuronal Overexpression ofFrontiers in genetics · 2019Article
- Review
- Post-transcriptional regulation of myotube elongation and myogenesis by Hoi Polloi.Development (Cambridge, England) · 2013Article
- Centralspindlin: at the heart of cytokinesis.Cytoskeleton (Hoboken, N.J.) · 2012Review
- MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function.Nature · 2012Article
- A barley ROP GTPase ACTIVATING PROTEIN associates with microtubules and regulates entry of the barley powdery mildew fungus into leaf epidermal cells.The Plant cell · 2011Article
- Expression of P2Y(6) receptors in the developing mouse skeletal muscle and after injury and repair.Journal of anatomy · 2011Article
- Genetic interaction of centrosomin and bazooka in apical domain regulation in Drosophila photoreceptor.PloS one · 2011Article
- Dysferlin interacts with histone deacetylase 6 and increases alpha-tubulin acetylation.PloS one · 2011Article
- Born to run: creating the muscle fiber.Current opinion in cell biology · 2010Review
- Cytokinesis proteins Tum and Pav have a nuclear role in Wnt regulation.Journal of cell science · 2010Article
- Cytoskeletal remodeling during myotube assembly and guidance: coordinating the actin and microtubule networks.Communicative & integrative biology · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The microtubule (MT) cytoskeleton is reorganized during myogenesis as individual myoblasts fuse into multinucleated myotubes. Although this reorganization has long been observed in cell culture, these findings have not been validated during development, and proteins that regulate this process are largely unknown. We have identified a novel postmitotic function for the cytokinesis proteins RacGAP50C (Tumbleweed) and Pavarotti as essential regulators of MT organization during Drosophila myogenesis. We show that the localization of the MT nucleator gamma-tubulin changes from diffuse cytoplasmic staining in mononucleated myoblasts to discrete cytoplasmic puncta at the nuclear periphery in multinucleated myoblasts, and that this change in localization depends on RacGAP50C. RacGAP50C and gamma-tubulin colocalize at perinuclear sites in myotubes, and in RacGAP50C mutants gamma-tubulin remains dispersed throughout the cytoplasm. Furthermore, we show that the mislocalization of RacGAP50C in pavarotti mutants is sufficient to redistribute gamma-tubulin to the muscle fiber ends. Finally, myotubes in RacGAP50C mutants have MTs with non-uniform polarity, resulting in multiple guidance errors. Taken together, these findings provide strong evidence that the reorganization of the MT network that has been observed in vitro plays an important role in myotube extension and muscle patterning in vivo, and also identify two molecules crucial for this process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.