ArticleBiochemistry2009
Interactions between small heat shock protein alpha-crystallin and galectin-related interfiber protein (GRIFIN) in the ocular lens.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- The Mammalian Ocular Lens in Focus: Development, Anatomy, Physiology, Transparency, Biomechanics, and Age-related Challenges.Journal of visualized experiments : JoVE · 2026Review
- Through theGenes · 2024Review
- Soluble overexpression and purification of infectious bursal disease virus capsid protein VP2 inCell cycle (Georgetown, Tex.) · 2022Article
- Examining Galectin Gene Regulation by Reporter Assays.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Transcriptomics Analysis of Lens from Patients with Posterior Subcapsular Congenital Cataract.Genes · 2021Article
- Structural insight into the binding of human galectins to corneal keratan sulfate, its desulfated form and related saccharides.Scientific reports · 2020Article
- How galectins have become multifunctional proteins.Histology and histopathology · 2020Review
- Comparative Transcriptome Profiling of the LoachesFrontiers in genetics · 2019Article
- Article
- Identification of peptides in human Hsp20 and Hsp27 that possess molecular chaperone and anti-apoptotic activities.The Biochemical journal · 2015Article
- Vascular dysfunction precedes hypertension associated with a blood pressure locus on rat chromosome 12.American journal of physiology. Heart and circulatory physiology · 2014Article
- Matricellular proteins in the trabecular meshwork: review and update.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics · 2014Review
- HspB1, HspB5 and HspB4 in Human Cancers: Potent Oncogenic Role of Some of Their Client Proteins.Cancers · 2014Article
- Article
- Histone posttranslational modifications and cell fate determination: lens induction requires the lysine acetyltransferases CBP and p300.Nucleic acids research · 2013Article
- Novel roles for α-crystallins in retinal function and disease.Progress in retinal and eye research · 2012Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
As a member of the small heat shock protein superfamily, alpha-crystallin has a chaperone-like ability to recognize and bind denatured or unfolded proteins and prevent their aggregation. Recent studies suggest that alpha-crystallin may also interact with a variety of proteins under native conditions in vitro. To identify potential binding partners for alpha-crystallin in the intact ocular lens, we conducted cross-linking studies in transgenic mouse lenses designed for overexpression of His-tagged human alphaA-crystallin. Interacting proteins were copurified with the epitope-tagged crystallin complexes and were identified by tandem mass spectrometry. This approach identified GRIFIN (galectin-related interfiber protein) as a novel binding partner. Consistent with results from cross-linking, GRIFIN subunits copurified with alpha-crystallin complexes during size exclusion chromatography of nontransgenic mouse lens extracts prepared without chemical cross-linking. Equilibrium binding to GRIFIN was studied using native alpha-crystallin isolated from calf lenses as well as oligomeric complexes reconstituted from recombinant alphaA- and alphaB-crystallin subunits. Calf lens alpha-crystallin binds GRIFIN with relatively high affinity (K(d) = 6.5 +/- 0.8 microM) at a stoichiometry of 0.25 +/- 0.01 GRIFIN monomer/alpha-crystallin subunit. The binding interaction between alpha-crystallin and GRIFIN is enhanced up to 5-fold in the presence of 3 mM ATP. These binding data support the hypothesis that GRIFIN is a novel binding partner of alpha-crystallin in the lens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.