ArticleIn vitro cellular & developmental biology. Animal2009
Proteasome activity in a naïve mosquito cell line infected with Wolbachia pipientis wAlbB.
Article in In vitro cellular & developmental biology. Animal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- Remodeling of host lipid metabolism by Wolbachia strain wAlbB is associated with lipid accumulation and cardiolipin dysregulation in the Aedes aegypti fat body.Parasites & vectors · 2026Article
- Generation of Monoclonal Cultures from Wolbachia-infected Drosophila melanogaster JW18 Cell Line.Journal of visualized experiments : JoVE · 2025Article
- A Light in the Dark: Uncovering Wolbachia-Host Interactions Using Fluorescence Imaging.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Differences in proteome perturbations caused by the Wolbachia strain wAu suggest multiple mechanisms of Wolbachia-mediated antiviral activity.Scientific reports · 2023Article
- Mitotically inactivated mosquito cells support robust Wolbachia infection and replication.In vitro cellular & developmental biology. Animal · 2022Article
- From Mosquito Ovaries to Ecdysone; from Ecdysone toInsects · 2022Review
- Two Newly IntroducedApplied and environmental microbiology · 2021Article
- Growth and Maintenance ofInsects · 2021Review
- Comprehensive Quantitative Proteome Analysis ofFrontiers in physiology · 2021Article
- Dual proteomics of Drosophila melanogaster hemolymph infected with the heritable endosymbiont Spiroplasma poulsonii.PloS one · 2021Article
- Wolbachia endosymbionts subvert the endoplasmic reticulum to acquire host membranes without triggering ER stress.PLoS neglected tropical diseases · 2019Article
- TheMicrobiology spectrum · 2019Review
- Conditions facilitating infection of mosquito cell lines with Wolbachia, an obligate intracellular bacterium.In vitro cellular & developmental biology. Animal · 2019Article
- Whole genome screen reveals a novel relationship between Wolbachia levels and Drosophila host translation.PLoS pathogens · 2018Article
- Proteomic analysis of a mosquito host cell response to persistent Wolbachia infection.Research in microbiology · 2017Article
- Reliance ofGenetics · 2017Article
- Article
- Wolbachia Endosymbionts Modify Drosophila Ovary Protein Levels in a Context-Dependent Manner.Applied and environmental microbiology · 2016Article
- Native Wolbachia from Aedes albopictus Blocks Chikungunya Virus Infection In Cellulo.PloS one · 2015Article
- Proteomic profiling of a robust Wolbachia infection in an Aedes albopictus mosquito cell line.Molecular microbiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We used Wolbachia pipientis strain wAlbB from Aedes albopictus Aa23 cells to infect clonal Ae. albopictus TK-6 cells, which are resistant to 5-bromodeoxyuridine. Infected TK-6 cells were cultured in medium containing 5-bromodeoxyuridine to select against Aa23 cells that might have persisted in the inoculum. Infected TK-6 lines retained the Wolbachia infection for 5 mo, indicating that their metabolic processes support Wolbachia growth and multiplication. To investigate early events after Wolbachia infection, we labeled infected cells with (35)S[methionine/cysteine]. Patterns of labeled proteins on sodium dodecyl sulfate gels were similar in control and infected cells, with the exception of a 29-kDa protein. Tandem mass spectrometry revealed that the 29-kDa band included alpha and beta subunits of the 26S proteasome. Independent confirmation of the up-regulation of the proteasome was established by probing Western blots with a monoclonal antibody to the proteasome-associated co-factor, ubiquitin. Wolbachia's loss of metabolic pathways for the synthesis of most amino acids and retention of pathways for their uptake and metabolism suggest that proteasome activation provides a mechanism whereby controlled degradation of intracellular host proteins would increase availability of amino acids to support establishment and maintenance of the Wolbachia infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.