ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2009
Diffusion-weighted magnetic resonance imaging reversal by gene knockdown of matrix metalloproteinase-9 activities in live animal brains.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Trial
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- Imaging rhodopsin degeneration in vivo in a new model of ocular ischemia in living mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Article
- Anatomical, functional and molecular biomarker applications of magnetic resonance neuroimaging.Future neurology · 2015Article
- NIH workshop on clinical translation of molecular imaging probes and technology--meeting report.Molecular imaging and biology · 2014Article
- MRI reveals differential effects of amphetamine exposure on neuroglia in vivo.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Sodium sulfide prevents water diffusion abnormality in the brain and improves long term outcome after cardiac arrest in mice.Resuscitation · 2012Article
- Molecular imaging of atherosclerosis for improving diagnostic and therapeutic development.Circulation research · 2012Review
- Noninvasive detection of neural progenitor cells in living brains by MRI.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Inhaled nitric oxide improves outcomes after successful cardiopulmonary resuscitation in mice.Circulation · 2011Article
- DNA-based MRI probes for specific detection of chronic exposure to amphetamine in living brains.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The involvement of matrix metalloproteinase-9 (MMP-9) activities in the development of abnormal water diffusion in the brain after cardiac arrest is not fully understood. We used magnetic resonance imaging to determine the correlation between MMP-9 activity and the mechanism of abnormal water diffusion after global cerebral ischemia (GCI)-induced brain damage in C57black6 mice. We induced GCI in mice by occluding both carotid arteries for 60 min, then allowing reperfusion. We labeled a short DNA that targets mmp-9 mRNA activity [phosphorothioate-modified oligodeoxynucleotide (sODN)-mmp9] or a control probe without intracellular target (sODN-Ran) with iron-based MR contrast agent [superparamagnetic iron oxide nanoparticle (SPION)-mmp9 or SPION-Ran] or fluorescein isothiocyanate (FITC)-sODN-mmp9 or FITC-sODN-Ran; we then delivered these probes by intracerebroventricular infusion or intraperitoneal injection within 3 h of reperfusion. At low dose (120 pmol/kg) the SPION-mmp9 probe was retained at significant levels in the striatum and cortex of living brains 10 h after GCI. Probe retention was validated by similar elevation of mmp-9 mRNA and antigens in postmortem samples taken from regions that exhibited GCI-induced hyperintensity in diffusion-weighted imaging, and a significant reduction in apparent diffusion coefficient (rADC, p = 0.0006, n = 12). At a higher dose (120 nmol/kg), the FITC-sODN-mmp9 probe revealed significant knockdown of MMP-9 activity, per zymography, and a reversal of striatal rADC (p = 0.004, n = 6). These observations were not duplicated in the control group. We conclude that expression of mmp-9 mRNA is associated with abnormal ADC after GCI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.