ArticleWorld journal of gastroenterology2009
Ex-vivo evaluation of gene therapy vectors in human pancreatic (cancer) tissue slices.
Article in World journal of gastroenterology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Relevance of humanized three-dimensional tumor tissue models: a descriptive systematic literature review.Cellular and molecular life sciences : CMLS · 2020Pooled it
- Metabolic Salvage and Acyl-chain Remodeling Support Glycosphingolipid Synthesis within the PDAC Tumor Microenvironment.bioRxiv : the preprint server for biology · 2026Article
- Precision-cut tumor slices: a biomimetic tool for accelerating oncological development from basic research to clinical translation.Frontiers in physiology · 2026Review
- Ex vivo functional whole organ in biomedical research: a review.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2025Review
- Does the Microenvironment Hold the Hidden Key for Functional Precision Medicine in Pancreatic Cancer?Cancers · 2021Review
- Article
- Tumour-reprogrammed stromal BCAT1 fuels branched-chain ketoacid dependency in stromal-rich PDAC tumours.Nature metabolism · 2020Article
- Progress in Animal Models of Pancreatic Ductal Adenocarcinoma.Journal of Cancer · 2020Review
- Ex vivo organotypic culture system of precision-cut slices of human pancreatic ductal adenocarcinoma.Scientific reports · 2019Article
- Use of Precision-Cut Lung Slices as anMolecular therapy. Methods & clinical development · 2018Article
- Pancreas 3D Organoids: Current and Future Aspects as a Research Platform for Personalized Medicine in Pancreatic Cancer.Cellular and molecular gastroenterology and hepatology · 2018Review
- Human Organ Culture: Updating the Approach to Bridge the Gap fromFrontiers in medicine · 2017Review
- Ex Vivo Testing of Patient-Derived Xenografts Mirrors the Clinical Outcome of Patients with Pancreatic Ductal Adenocarcinoma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2016Article
- Modeling pancreatic cancer with organoids.Trends in cancer · 2016Article
- Organotypic culture of breast tumor explants as a multicellular system for the screening of natural compounds with antineoplastic potential.BioMed research international · 2015Article
- Sensitization of pancreatic cancer to chemoradiation by the Chk1 inhibitor MK8776.Clinical cancer research : an official journal of the American Association for Cancer Research · 2013Article
- An in vitro model of human acute ethanol exposure that incorporates CXCR3- and CXCR4-dependent recruitment of immune cells.Toxicological sciences : an official journal of the Society of Toxicology · 2013Article
- Article
- Frontiers in Suicide Gene Therapy of Cancer.Journal of genetic syndromes & gene therapy · 2012Article
- Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment.OncoimmunologyArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo culture human pancreatic tissue obtained from small resection specimens as a pre-clinical model for examining virus-host interactions.
methodsHuman pancreatic tissue samples (malignant and normal) were obtained from surgical specimens and processed immediately to tissue slices. Tissue slices were cultured ex vivo for 1-6 d in an incubator using 95% O(2). Slices were subsequently analyzed for viability and morphology. In addition the slices were incubated with different viral vectors expressing the reporter genes GFP or DsRed. Expression of these reporter genes was measured at 72 h after infection.
resultsWith the Krumdieck tissue slicer, uniform slices could be generated from pancreatic tissue but only upon embedding the tissue in 3% low melting agarose. Immunohistological examination showed the presence of all pancreatic cell types. Pancreatic normal and cancer tissue slices could be cultured for up to 6 d, while retaining viability and a moderate to good morphology. Reporter gene expression indicated that the slices could be infected and transduced efficiently by adenoviral vectors and by adeno associated viral vectors, whereas transduction with lentiviral vectors was limited. For the adenoviral vector, the transduction seemed limited to the peripheral layers of the explants.
conclusionThe presented system allows reproducible processing of minimal amounts of pancreatic tissue into slices uniform in size, suitable for pre-clinical evaluation of gene therapy vectors.
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Registered trials
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