ArticleMolecular systems biology2009
Force- and kinesin-8-dependent effects in the spatial regulation of fission yeast microtubule dynamics.
Article in Molecular systems biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 108 citations in OpenAlex.
- Branching, crosslinking, and decentralization of microtubules accelerates intracellular assembly.Biophysical journal · 2025Article
- EB3-informed dynamics of the microtubule stabilizing cap during stalled growth.Biophysical journal · 2025Article
- The motor domain of the kinesin Kip2 promotes microtubule polymerization at microtubule tips.The Journal of cell biology · 2023Article
- Reliable and robust control of nucleus centering is contingent on nonequilibrium force patterns.iScience · 2023Article
- Fission yeast Dis1 is an unconventional TOG/XMAP215 that induces microtubule catastrophe to drive chromosome pulling.Communications biology · 2022Article
- Stochastic model of T cell repolarization during target elimination (II).Biophysical journal · 2022Article
- Article
- Microtubule-associated proteins and motors required for ectopic microtubule array formation in Saccharomyces cerevisiae.Genetics · 2021Article
- Article
- Review
- The concerted actions of Tip1/CLIP-170, Klp5/Kinesin-8, and Alp14/XMAP215 regulate microtubule catastrophe at the cell end.Journal of molecular cell biology · 2019Article
- Kinesin-8 and Dis1/TOG collaborate to limit spindle elongation from prophase to anaphase A for proper chromosome segregation in fission yeast.Journal of cell science · 2019Article
- Pivoting of microtubules driven by minus-end-directed motors leads to spindle assembly.BMC biology · 2019Article
- Association of mitochondria with microtubules inhibits mitochondrial fission by precluding assembly of the fission protein Dnm1.The Journal of biological chemistry · 2019Article
- Opposing kinesin complexes queue at plus tips to ensure microtubule catastrophe at cell ends.EMBO reports · 2018Article
- Metaphase kinetochore movements are regulated by kinesin-8 motors and microtubule dynamic instability.Molecular biology of the cell · 2018Article
- Polymerisation force of a rigid filament bundle: diffusive interaction leads to sublinear force-number scaling.Scientific reports · 2018Article
- Contributions of Microtubule Dynamic Instability and Rotational Diffusion to Kinetochore Capture.Biophysical journal · 2017Article
- Kinesin-8 effects on mitotic microtubule dynamics contribute to spindle function in fission yeast.Molecular biology of the cell · 2016Article
- Microtubule organization is determined by the shape of epithelial cells.Nature communications · 2016Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubules (MTs) are central to the organisation of the eukaryotic intracellular space and are involved in the control of cell morphology. For these purposes, MT polymerisation dynamics are tightly regulated. Using automated image analysis software, we investigate the spatial dependence of MT dynamics in interphase fission yeast cells with unprecedented statistical accuracy. We find that MT catastrophe frequencies (switches from polymerisation to depolymerisation) strongly depend on intracellular position. We provide evidence that compressive forces generated by MTs growing against the cell pole locally reduce MT growth velocities and enhance catastrophe frequencies. Furthermore, we find evidence for an MT length-dependent increase in the catastrophe frequency that is mediated by kinesin-8 proteins (Klp5/6). Given the intrinsic susceptibility of MT dynamics to compressive forces and the widespread importance of kinesin-8 proteins, we propose that similar spatial regulation of MT dynamics plays a role in other cell types as well. In addition, our systematic and quantitative data should provide valuable input for (mathematical) models of MT organisation in living cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.