ArticleMolecular systems biology2009
Temporal switching and cell-to-cell variability in Ca2+ release activity in mammalian cells.
Article in Molecular systems biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Heat-hypersensitive mutants of ryanodine receptor type 1 revealed by microscopic heating.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Essential Roles of Natural Products and Gaseous Mediators on Neuronal Cell Death or Survival.International journal of molecular sciences · 2016Review
- Endothelial cells decode VEGF-mediated Ca2+ signaling patterns to produce distinct functional responses.Science signaling · 2016Article
- Variability of Gene Expression Identifies Transcriptional Regulators of Early Human Embryonic Development.PLoS genetics · 2015Article
- A Systemized Approach to Investigate Ca(2+) Synchronization in Clusters of Human Induced Pluripotent Stem-Cell Derived Cardiomyocytes.Frontiers in cell and developmental biology · 2015Article
- Microfluidic interrogation and mathematical modeling of multi-regime calcium signaling dynamics.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Quantitative biology of single neurons.Journal of the Royal Society, Interface · 2012Review
- A network-oriented perspective on cardiac calcium signaling.American journal of physiology. Cell physiology · 2012Review
- Imaging single-cell signaling dynamics with a deterministic high-density single-cell trap array.Analytical chemistry · 2011Article
- The effect of coupled stochastic processes in a two-state biochemical switch.Journal of biological physics · 2011Article
- Hi-Fi transmission of periodic signals amid cell-to-cell variability.Molecular bioSystems · 2011Article
- High-throughput analysis of calcium signalling kinetics in astrocytes stimulated with different neurotransmitters.PloS one · 2011Article
- Variability in G-protein-coupled signaling studied with microfluidic devices.Biophysical journal · 2010Article
- Calcium signals driven by single channel noise.PLoS computational biology · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetically identical cells in a uniform external environment can exhibit different phenotypes, which are often masked by conventional measurements that average over cell populations. Although most studies on this topic have used microorganisms, differentiated mammalian cells have rarely been explored. Here, we report that only approximately 40% of clonal human embryonic kidney 293 cells respond with an intracellular Ca(2+) increase when ryanodine receptor Ca(2+) release channels in the endoplasmic reticulum are maximally activated by caffeine. On the other hand, the expression levels of ryanodine receptor showed a unimodal distribution. We showed that the difference in the caffeine sensitivity depends on a critical balance between Ca(2+) release and Ca(2+) uptake activities, which is amplified by the regenerative nature of the Ca(2+) release mechanism. Furthermore, individual cells switched between the caffeine-sensitive and caffeine-insensitive states with an average transition time of approximately 65 h, suggestive of temporal fluctuation in endogenous protein expression levels associated with caffeine response. These results suggest the significance of regenerative mechanisms that amplify protein expression noise and induce cell-to-cell phenotypic variation in mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.