ArticleProceedings of the National Academy of Sciences of the United States of America2009
Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 79 citations in OpenAlex.
- Non-canonical cytochrome P450 enzymes expand the diversity of bacterial hemoproteins.Nature chemical biology · 2026Article
- Discovery of noncanonical cytochrome P450 enzymes in nature.Nature chemical biology · 2026Article
- Photosensitizer Repositioning Affords an Enantiocomplementary Enzyme for [2 + 2]-Cycloadditions.Angewandte Chemie (International ed. in English) · 2025Article
- Non-Canonical Cytochrome P450 Enzymes in Nature.bioRxiv : the preprint server for biology · 2024Article
- Noncanonical Amino Acids in Biocatalysis.Chemical reviews · 2024Review
- Heme Spin Distribution in the Substrate-Free and Inhibited Novel CYP116B5hd: A Multifrequency Hyperfine Sublevel Correlation (HYSCORE) Study.Molecules (Basel, Switzerland) · 2024Article
- The role of basicity in selective C-H bond activation by transition metal-oxidos.Dalton transactions (Cambridge, England : 2003) · 2023Review
- A haem-sequestering plant peptide promotes iron uptake in symbiotic bacteria.Nature microbiology · 2022Article
- A Noncanonical Tryptophan Analogue Reveals an Active Site Hydrogen Bond Controlling Ferryl Reactivity in a Heme Peroxidase.JACS Au · 2021Article
- Rewiring the "Push-Pull" Catalytic Machinery of a Heme Enzyme Using an Expanded Genetic Code.ACS catalysis · 2020Article
- Carbon-Based Oxamate Cobalt(III) Complexes as Bioenzyme Mimics for Contaminant Elimination in High Backgrounds of Complicated Constituents.Materials (Basel, Switzerland) · 2017Article
- Characterization of a selenocysteine-ligated P450 compound I reveals direct link between electron donation and reactivity.Nature chemistry · 2017Article
- Enzymatic catalysis: New functional twists for P450s.Nature chemistry · 2017Article
- A synthetic tRNA for EF-Tu mediated selenocysteine incorporation in vivo and in vitro.FEBS letters · 2015Article
- Harnessing selenocysteine reactivity for oxidative protein folding.Chemical science · 2015Article
- Structural and functional characterization of a cytochrome P450 2B4 F429H mutant with an axial thiolate-histidine hydrogen bond.Biochemistry · 2014Article
- Enhancing the efficiency and regioselectivity of P450 oxidation catalysts by unnatural amino acid mutagenesis.Chembiochem : a European journal of chemical biology · 2014Article
- Site-specific insertion of selenium into the redox-active disulfide of the flavoprotein augmenter of liver regeneration.Archives of biochemistry and biophysics · 2014Article
- A tale of two toxicities: malformed selenoproteins and oxidative stress both contribute to selenium stress in plants.Annals of botany · 2013Review
- Rewiring translation for elongation factor Tu-dependent selenocysteine incorporation.Angewandte Chemie (International ed. in English) · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unique monooxygenase activity of cytochrome P450cam has been attributed to coordination of a cysteine thiolate to the heme cofactor. To investigate this interaction, we replaced cysteine with the more electron-donating selenocysteine. Good yields of the selenoenzyme were obtained by bacterial expression of an engineered gene containing the requisite UGA codon for selenocysteine and a simplified yet functional selenocysteine insertion sequence (SECIS). The sulfur-to-selenium substitution subtly modulates the structural, electronic, and catalytic properties of the enzyme. Catalytic activity decreases only 2-fold, whereas substrate oxidation becomes partially uncoupled from electron transfer, implying a more complex role for the axial ligand than generally assumed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.