ArticleThe Plant cell2009
Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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44 citing papers in PubMed, 81 citations in OpenAlex.
- A plastid carbohydrate carrier mediates ribose recycling from nucleotide catabolism and glucose export from starch degradation.Nature communications · 2025Article
- A monomer-dimer switch modulates the activity of plant adenosine kinase.Journal of experimental botany · 2025Article
- Identification of novel candidate genes for regulating oil composition in soybean seeds under environmental stresses.Frontiers in plant science · 2025Article
- A cell wall-localized cytokinin/purine riboside nucleosidase is involved in apoplastic cytokinin metabolism inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Pyrimidine catabolism is required to prevent the accumulation of 5-methyluridine in RNA.Nucleic acids research · 2023Article
- A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis.The Plant cell · 2023Article
- Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings.Frontiers in plant science · 2023Article
- Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules.Nature communications · 2022Article
- Initiation of cytosolic plant purine nucleotide catabolism involves a monospecific xanthosine monophosphate phosphatase.Nature communications · 2021Article
- Review
- Enhanced nucleotide analysis enables the quantification of deoxynucleotides in plants and algae revealing connections between nucleoside and deoxynucleoside metabolism.The Plant cell · 2021Article
- Nucleoside Metabolism Is Induced in Common Bean During Early Seedling Development.Frontiers in plant science · 2021Article
- The genetic framework of shoot regeneration in Arabidopsis comprises master regulators and conditional fine-tuning factors.Communications biology · 2020Article
- A Kinase and a Glycosylase Catabolize Pseudouridine in the Peroxisome to Prevent Toxic Pseudouridine Monophosphate Accumulation.The Plant cell · 2020Article
- WHITE STRIPE LEAF8, encoding a deoxyribonucleoside kinase, is involved in chloroplast development in rice.Plant cell reports · 2020Article
- Nucleotide Metabolism in Plants.Plant physiology · 2020Article
- Pyrimidine Salvage: Physiological Functions and Interaction with Chloroplast Biogenesis.Plant physiology · 2019Article
- Phytohormone metabolism in human cells: Cytokinins are taken up and interconverted in HeLa cell culture.FASEB bioAdvances · 2019Article
- AMP and GMP Catabolism in Arabidopsis Converge on Xanthosine, Which Is Degraded by a Nucleoside Hydrolase Heterocomplex.The Plant cell · 2019Article
- Cytokinin Transporters: Multisite Players in Cytokinin Homeostasis and Signal Distribution.Frontiers in plant science · 2019Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleoside degradation and salvage are important metabolic pathways but hardly understood in plants. Recent work on human pathogenic protozoans like Leishmania and Trypanosoma substantiates an essential function of nucleosidase activity. Plant nucleosidases are related to those from protozoans and connect the pathways of nucleoside degradation and salvage. Here, we describe the cloning of such an enzyme from Arabidopsis thaliana, Uridine-Ribohydrolase 1 (URH1) and the characterization by complementation of a yeast mutant. Furthermore, URH1 was synthesized as a recombinant protein in Escherichia coli. The pure recombinant protein exhibited highest hydrolase activity for uridine, followed by inosine and adenosine, the corresponding K(m) values were 0.8, 1.4, and 0.7 mM, respectively. In addition, URH1 was able to cleave the cytokinin derivative isopentenyladenine-riboside. Promoter beta-glucuronidase fusion studies revealed that URH1 is mainly transcribed in the vascular cells of roots and in root tips, guard cells, and pollen. Mutants expressing the Arabidopsis enzyme or the homolog from rice (Oryza sativa) exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid, providing clear evidence for a pivotal function of URH1 as regulative in pyrimidine degradation. Moreover, mutants with increased and decreased nucleosidase activity are delayed in germination, indicating that this enzyme activity must be well balanced in the early phase of plant development.
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