ArticleBMC plant biology2009
The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway.
Article in BMC plant biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 114 citations in OpenAlex.
- Advances in the Function Roles of Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferases: A Key Enzyme Linking Phenylpropanoid Metabolism to Plant Terrestrial Adaptation.Plants (Basel, Switzerland) · 2026Review
- Genome-wide analysis of BAHD gene family and in vivo characterization of HQT-like genes involved in chlorogenic acid synthesis in tobacco (Nicotiana tabacum L.).BMC plant biology · 2026Article
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- Medicinal Plants for a Healthy Gut Microbiome: Scientific Insights into Modern Herbal Applications.International journal of molecular sciences · 2025Review
- Targeted metabolomic and transcriptomic analyses provide insights into flavonoid biosynthesis in the grain of Foxtail millet.BMC genomics · 2025Article
- CYP98A monooxygenases: a key enzyme family in plant phenolic compound biosynthesis.Horticulture research · 2025Article
- Integrative analysis of genome-wide association studies of polyphenols in apple fruits identifies the MdDof2.4Horticulture research · 2025Article
- Investigation of the association between gene expression levels and phenolic compound content in the leaves ofHeliyon · 2024Article
- Risk Assessment of Chlorogenic and Isochlorogenic Acids in Coffee By-Products.Molecules (Basel, Switzerland) · 2023Review
- Identification ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Effect of Stress Signals and Ib-rolB/C Overexpression on Secondary Metabolite Biosynthesis in Cell Cultures of Ipomoea batatasInternational journal of molecular sciences · 2022Article
- The Population Structure of a Globe Artichoke Worldwide Collection, as Revealed by Molecular and Phenotypic Analyzes.Frontiers in plant science · 2022Article
- Transcriptome-Wide Identification and Quantification of Caffeoylquinic Acid Biosynthesis Pathway and Prediction of Its Putative BAHDs Gene Complex inInternational journal of molecular sciences · 2021Article
- Article
- A GDSL lipase-like from Ipomoea batatas catalyzes efficient production of 3,5-diCQA when expressed in Pichia pastoris.Communications biology · 2020Article
- Changes in Phenolics and Fatty Acids Composition and Related Gene Expression during the Development from Seed to Leaves of Three Cultivated Cardoon Genotypes.Antioxidants (Basel, Switzerland) · 2020Article
- Integrated Analysis of the Transcriptome and Metabolome ofInternational journal of molecular sciences · 2020Article
- Hybrid transcriptome sequencing approach improved assembly and gene annotation in Cynara cardunculus (L.).BMC genomics · 2020Article
- Identification and Functional Characterization of Genes Involved in the Biosynthesis of Caffeoylquinic Acids in Sunflower (Frontiers in plant science · 2019Article
- Correlation of the temporal and spatial expression patterns of HQT with the biosynthesis and accumulation of chlorogenic acid inHorticulture research · 2019Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe leaves of globe artichoke and cultivated cardoon (Cynara cardunculus L.) have significant pharmaceutical properties, which mainly result from their high content of polyphenolic compounds such as monocaffeoylquinic and dicaffeoylquinic acid (DCQ), and a range of flavonoid compounds.
resultsHydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase (HQT) encoding genes have been isolated from both globe artichoke and cultivated cardoon (GenBank accessions DQ915589 and DQ915590, respectively) using CODEHOP and PCR-RACE. A phylogenetic analysis revealed that their sequences belong to one of the major acyltransferase groups (anthranilate N-hydroxycinnamoyl/benzoyltransferase). The heterologous expression of globe artichoke HQT in E. coli showed that this enzyme can catalyze the esterification of quinic acid with caffeoyl-CoA or p-coumaroyl-CoA to generate, respectively, chlorogenic acid (CGA) and p-coumaroyl quinate. Real time PCR experiments demonstrated an increase in the expression level of HQT in UV-C treated leaves, and established a correlation between the synthesis of phenolic acids and protection against damage due to abiotic stress. The HQT gene, together with a gene encoding hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase (HCT) previously isolated from globe artichoke, have been incorporated within the developing globe artichoke linkage maps.
conclusionA novel acyltransferase involved in the biosynthesis of CGA in globe artichoke has been isolated, characterized and mapped. This is a good basis for our effort to understand the genetic basis of phenylpropanoid (PP) biosynthesis in C. cardunculus.
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