ArticleBMC biotechnology2009
Chromato-panning: an efficient new mode of identifying suitable ligands from phage display libraries.
Article in BMC biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Review
- Upgrading of bio-separation and bioanalysis using synthetic polymers: Molecularly imprinted polymers (MIPs), cryogels, stimuli-responsive polymers.Engineering in life sciences · 2022Review
- Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2019Article
- Isolation of Antibodies to Heparan Sulfate on Glypicans by Phage Display.Current protocols in protein science · 2018Article
- Naïve Human Antibody Libraries for Infectious Diseases.Advances in experimental medicine and biology · 2017Review
- The isolation of novel phage display-derived human recombinant antibodies against CCR5, the major co-receptor of HIV.Viral immunology · 2013Article
- Techniques for molecular imaging probe design.Molecular imaging · 2011Review
- Peptide phage display as a tool for drug discovery: targeting membrane receptors.Molecules (Basel, Switzerland) · 2011Review
- Phage display in molecular imaging and diagnosis of cancer.Chemical reviews · 2010Review
- Progress in phage display: evolution of the technique and its application.Cellular and molecular life sciences : CMLS · 2010Review
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6 authors.
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Abstract
backgroundPhage Display technology is a well established technique for high throughput screening of affinity ligands. Here we describe a new compact chromato-panning procedure for selection of suitable binders from a phage peptide display library.
resultsBoth phages and E. coli cells pass non-hindered through the interconnected pores of macroporous gel, so called cryogel. After coupling a ligand to a monolithic cryogel column, the phage library was applied on the column and non-bound phages were washed out. The selection of strong phage-binders was achieved already after the first panning cycle due to the efficient separation of phage-binders from phage-non-binders in chromatographic mode rather than in batch mode as in traditional biopanning procedures. E. coli cells were applied on the column for infection with the specifically bound phages.
conclusionChromato-panning allows combining several steps of the panning procedure resulting in 4-8 fold decrease of total time needed for phage selection.
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