ArticleMolecular biotechnology2009
Development of a RT real-time PCR for the detection and quantification of human rhinoviruses.
Article in Molecular biotechnology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- Epidemiological and molecular characteristics of human parainfluenza virus in southern China during 2016-2020.Virologica Sinica · 2025Article
- An outbreak of rhinovirus infection in a primary school in Shenyang City, China, in 2022.Biosafety and health · 2024Article
- Seasonal Dynamics of the Airborne Bacterial Community and Selected Viruses in a Children's Daycare Center.PloS one · 2016Article
- Duration of rhinovirus shedding in the upper respiratory tract in the first year of life.Pediatrics · 2014Article
- Etiology and clinical outcomes of acute respiratory virus infection in hospitalized adults.Infection & chemotherapy · 2014Article
- Simultaneous detection and differentiation of human rhino- and enteroviruses in clinical specimens by real-time PCR with locked nucleic Acid probes.Journal of clinical microbiology · 2013Article
- Use of an improved quantitative polymerase chain reaction assay to determine differences in human rhinovirus viral loads in different populations.Diagnostic microbiology and infectious disease · 2012Article
- One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3.BMC veterinary research · 2012Article
- Development of a quantitative real-time nucleic acid sequence-based amplification assay with an internal control using molecular beacon probes for selective and sensitive detection of human rhinovirus serotypes.Molecular biotechnology · 2012Article
- Article
- Comparison of the FilmArray Respiratory Panel and Prodesse real-time PCR assays for detection of respiratory pathogens.Journal of clinical microbiology · 2011Article
- Rhinovirus frequently detected in elderly adults attending an emergency department.Journal of medical virology · 2011Article
- Epidemic viral pneumonia and other emerging pathogens.Clinics in chest medicine · 2011Review
- Detection of human rhinoviruses in the lower respiratory tract of lung transplant recipients.Archives of virology · 2011Article
- Improvement of HRV quantification using cRNA-based standards for real time RT-PCR.Molecular biotechnology · 2011Article
- Frequency of viral etiology in symptomatic adult upper respiratory tract infections.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human Rhinoviruses (HRV) are the most common viral agents, being responsible for upper as well as lower respiratory tract infections. Evidence demonstrating that HRV disease is not exclusively limited to the upper airways and may cause lower respiratory complications, together with the frequency of HRV infections and the increasing number of immunocompromised patients underline the need for including HRV in virological diagnostics of acute lower respiratory tract illness. This article describes the development and optimization of a reverse transcription (RT) real-time PCR assay for quantification of HRV RNA in clinical samples. Efficiency, sensitivity, specificity, inter- and intra-assay variability, and dynamic range have been determined. Subsequently, the assay has been validated on bronchoalveolar lavage (BAL) specimens obtained from immunocompetent and immunocompromised patients.
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