ArticleNano letters2009
Lighting up individual DNA binding proteins with quantum dots.
Article in Nano letters, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 49 citations in OpenAlex.
- Time for NanoNeuro.Nature methods · 2021Review
- A Parallelized Nanofluidic Device for High-Throughput Optical DNA Mapping of Bacterial Plasmids.Micromachines · 2021Article
- Electrophoretic stretching and imaging of single native chromatin fibers in nanoslits.Biomicrofluidics · 2017Article
- Distribution of distances between DNA barcode labels in nanochannels close to the persistence length.The Journal of chemical physics · 2015Article
- DNA combing on low-pressure oxygen plasma modified polysilsesquioxane substrates for single-molecule studies.Biomicrofluidics · 2014Article
- Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices.Nucleic acids research · 2014Article
- Toward single-molecule optical mapping of the epigenome.ACS nano · 2014Article
- Beyond gel electrophoresis: microfluidic separations, fluorescence burst analysis, and DNA stretching.Chemical reviews · 2013Review
- Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins.Angewandte Chemie (International ed. in English) · 2012Article
- Resolution limit for DNA barcodes in the Odijk regime.Biomicrofluidics · 2012Article
- Labeling DNA for single-molecule experiments: methods of labeling internal specific sequences on double-stranded DNA.Nanoscale · 2011Review
- Aromatic aldehyde and hydrazine activated peptide coated quantum dots for easy bioconjugation and live cell imaging.Bioconjugate chemistry · 2011Article
- Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping.Lab on a chip · 2011Article
- Supported lipid bilayers and DNA curtains for high-throughput single-molecule studies.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Peptide nucleic acids as tools for single-molecule sequence detection and manipulation.Nano letters · 2010Article
- Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point.Lab on a chip · 2010Article
- Fluorescent marker for direct detection of specific dsDNA sequences.Analytical chemistry · 2009Article
- Nanometer distance measurements between multicolor quantum dots.Nano letters · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
The ability to determine the precise loci and occupancy of DNA-binding proteins is instrumental to our understanding of cellular processes like gene expression and regulation. We propose a single-molecule approach for the direct visualization of proteins bound to their template DNA. Fluorescent quantum dots (QD) are used to label proteins bound to DNA, allowing multicolor, nanometer-resolution localization. Protein-DNA complexes are linearly extended and imaged to determine the precise location of the protein binding sites. The method is demonstrated by detecting individual QD-labeled T7-RNA polymerases on the T7 bacteriophage genome. This work demonstrates the potential of this approach to precisely read protein binding position or, alternatively, "write" such information on extended DNA with QDs via sequence-specific molecular recognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.