ArticleMolecular genetics and genomics : MGG2009
Insensitivity of chloroplast gene expression to DNA methylation.
Article in Molecular genetics and genomics : MGG, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Carboxylation and Oxygenation Kinetics and Large Subunit (rbcL) DNA Sequences for Rubisco From Two Ecotypes of Plantago lanceolata L. That Are Native to Sites Differing in Atmospheric COPlant, cell & environment · 2025Article
- PSTVd infection inFrontiers in plant science · 2023Article
- Integrative analysis of chloroplast DNA methylation in a marine alga-Saccharina japonica.Plant molecular biology · 2021Article
- An Insight Into the Mechanism of Plant Organelle Genome Maintenance and Implications of Organelle Genome in Crop Improvement: An Update.Frontiers in cell and developmental biology · 2021Review
- Chloroplast development and genomes uncoupled signaling are independent of the RNA-directed DNA methylation pathway.Scientific reports · 2020Article
- Article
- Polyploidization is accompanied by synonymous codon usage bias in the chloroplast genomes of both cotton and wheat.PloS one · 2020Article
- Comparative sequence and methylation analysis of chloroplast and amyloplast genomes from rice.Plant molecular biology · 2019Article
- Identification of the DNA methyltransferases establishing the methylome of the cyanobacterium Synechocystis sp. PCC 6803.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Engineering Triterpene and Methylated Triterpene Production in Plants Provides Biochemical and Physiological Insights into Terpene Metabolism.Plant physiology · 2016Article
- Evolutionary Stasis in Cycad Plastomes and the First Case of Plastome GC-Biased Gene Conversion.Genome biology and evolution · 2015Article
- Highly Iterated Palindromic Sequences (HIPs) and Their Relationship to DNA Methyltransferases.Life (Basel, Switzerland) · 2015Article
- Synonymous codon usage bias in plant mitochondrial genes is associated with intron number and mirrors species evolution.PloS one · 2015Article
- Synonymous Codon Usage Bias in the Plastid Genome is Unrelated to Gene Structure and Shows Evolutionary Heterogeneity.Evolutionary bioinformatics online · 2015Article
- Article
- Review
- Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A.Nucleic acids research · 2012Article
- Genome-wide analysis of plastid gene expression in potato leaf chloroplasts and tuber amyloplasts: transcriptional and posttranscriptional control.Plant physiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Presence and possible functions of DNA methylation in plastid genomes of higher plants have been highly controversial. While a number of studies presented evidence for the occurrence of both cytosine and adenine methylation in plastid genomes and proposed a role of cytosine methylation in the transcriptional regulation of plastid genes, several recent studies suggested that at least cytosine methylation may be absent from higher plant plastid genomes. To test if either adenine or cytosine methylation can play a regulatory role in plastid gene expression, we have introduced cyanobacterial genes for adenine and cytosine DNA methyltransferases (methylases) into the tobacco plastid genome by chloroplast transformation. Using DNA cleavage with methylation-sensitive and methylation-dependent restriction endonucleases, we show that the plastid genomes in the transplastomic plants are efficiently methylated. All transplastomic lines are phenotypically indistinguishable from wild-type plants and, moreover, show no alterations in plastid gene expression. Our data indicate that the expression of plastid genes is not sensitive to DNA methylation and, hence, suggest that DNA methylation is unlikely to be involved in the transcriptional regulation of plastid gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.