ArticlePloS one2009
An order of magnitude faster AIP1-associated actin disruption than nucleation by the Arp2/3 complex in lamellipodia.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- Force transmission by retrograde actin flow-induced dynamic molecular stretching of Talin.Nature communications · 2023Article
- Article
- Discrete mechanical model of lamellipodial actin network implements molecular clutch mechanism and generates arcs and microspikes.PLoS computational biology · 2021Article
- Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts.Scientific reports · 2021Article
- Catastrophic actin filament bursting by cofilin, Aip1, and coronin.The Journal of biological chemistry · 2020Article
- Real-Time Single-Molecule Kinetic Analyses of AIP1-Enhanced Actin Filament Severing in the Presence of Cofilin.Journal of molecular biology · 2019Article
- Convection-Induced Biased Distribution of Actin Probes in Live Cells.Biophysical journal · 2019Article
- Functions of actin-interacting protein 1 (AIP1)/WD repeat protein 1 (WDR1) in actin filament dynamics and cytoskeletal regulation.Biochemical and biophysical research communications · 2018Review
- AIP1 and cofilin ensure a resistance to tissue tension and promote directional cell rearrangement.Nature communications · 2018Article
- Helical rotation of the diaphanous-related formin mDia1 generates actin filaments resistant to cofilin.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Overview of Single-Molecule Speckle (SiMS) Microscopy and Its Electroporation-Based Version with Efficient Labeling and Improved Spatiotemporal Resolution.Sensors (Basel, Switzerland) · 2017Review
- Model of turnover kinetics in the lamellipodium: implications of slow- and fast- diffusing capping protein and Arp2/3 complex.Physical biology · 2016Article
- Single-molecule imaging of a three-component ordered actin disassembly mechanism.Nature communications · 2015Article
- Actin and endocytosis in budding yeast.Genetics · 2015Review
- A phenomenological density-scaling approach to lamellipodial actin dynamics(†).Interface focus · 2014Article
- Jasplakinolide reduces actin and tropomyosin dynamics during myofibrillogenesis.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Dynamic localization of G-actin during membrane protrusion in neuronal motility.Current biology : CB · 2013Article
- F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins.Nature cell biology · 2013Article
- Distributed actin turnover in the lamellipodium and FRAP kinetics.Biophysical journal · 2013Article
- Srv2/cyclase-associated protein forms hexameric shurikens that directly catalyze actin filament severing by cofilin.Molecular biology of the cell · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism of lamellipod actin turnover is still under debate. To clarify the intracellular behavior of the recently-identified actin disruption mechanism, we examined kinetics of AIP1 using fluorescent single-molecule speckle microscopy. AIP1 is thought to cap cofilin-generated actin barbed ends. Here we demonstrate a reduction in actin-associated AIP1 in lamellipodia of cells overexpressing LIM-kinase. Moreover, actin-associated AIP1 was rapidly abolished by jasplakinolide, which concurrently blocked the F-actin-cofilin interaction. Jasplakinolide also slowed dissociation of AIP1, which is analogous to the effect of this drug on capping protein. These findings provide in vivo evidence of the association of AIP1 with barbed ends generated by cofilin-catalyzed filament disruption. Single-molecule observation found distribution of F-actin-associated AIP1 throughout lamellipodia, and revealed even faster dissociation of AIP1 than capping protein. The estimated overall AIP1-associated actin disruption rate, 1.8 microM/s, was one order of magnitude faster than Arp2/3 complex-catalyzed actin nucleation in lamellipodia. This rate does not suffice the filament severing rate predicted in our previous high frequency filament severing-annealing hypothesis. Our data together with recent biochemical studies imply barbed end-preferred frequent filament disruption. Frequent generation of AIP1-associated barbed ends and subsequent release of AIP1 may be the mechanism that facilitates previously observed ubiquitous actin polymerization throughout lamellipodia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.