ArticleProceedings of the National Academy of Sciences of the United States of America2009
The DNA-damage effector checkpoint kinase 1 is essential for chromosome segregation and cytokinesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
57 citing papers in PubMed, 105 citations in OpenAlex.
- Review
- β-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division.Communications biology · 2026Article
- Article
- The histone H3.3 K27M mutation suppresses Ser31phosphorylation and mitotic fidelity, which can directly drive gliomagenesis.Current biology : CB · 2025Article
- Mis-splicing of Mitotic Regulators Sensitizes SF3B1-Mutated Human HSCs to CHK1 Inhibition.Blood cancer discovery · 2024Article
- Mcph1, mutated in primary microcephaly, is also crucial for erythropoiesis.EMBO reports · 2024Article
- CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos.EMBO reports · 2023Article
- Checkpoint Kinase 1 Is a Key Signal Transducer of DNA Damage in the Early Mammalian Cleavage Embryo.International journal of molecular sciences · 2023Review
- On the assembly of the mitotic spindle, bistability and hysteresis.Cellular and molecular life sciences : CMLS · 2023Article
- Chk1 inhibition-induced BRCAness synergizes with olaparib in p53-deficient cancer cells.Cell cycle (Georgetown, Tex.) · 2023Article
- Quantifying cell-cycle-dependent chromatin dynamics during interphase by live 3D tracking.iScience · 2022Article
- DNA damage repair: historical perspectives, mechanistic pathways and clinical translation for targeted cancer therapy.Signal transduction and targeted therapy · 2021Review
- Review
- Kv10.1 Regulates Microtubule Dynamics during Mitosis.Cancers · 2020Article
- Building bridges between chromosomes: novel insights into the abscission checkpoint.Cellular and molecular life sciences : CMLS · 2019Review
- Quantitative phosphoproteomics reveals mitotic function of the ATR activator ETAA1.The Journal of cell biology · 2019Article
- Src activation by Chk1 promotes actin patch formation and prevents chromatin bridge breakage in cytokinesis.The Journal of cell biology · 2018Article
- Genomic structure, expression, and functional characterization of checkpoint kinase 1 from Penaeus monodon.PloS one · 2018Article
- Checkpoint kinase 1-induced phosphorylation ofThe Journal of biological chemistry · 2017Article
- Checkpoint kinase 1 is essential for normal B cell development and lymphomagenesis.Nature communications · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Defective genome maintenance mechanisms, involving DNA repair and cell-cycle checkpoint pathways, initiate genetic instability in many sporadic and hereditary cancers. The DNA damage effector Checkpoint kinase 1 (Chk1) is a critical component of DNA replication, intra-S phase, and G(2)/M phase checkpoints and a recently reported mitotic spindle-assembly checkpoint. Here, we report for the first time that haploinsufficiency of Chk1 in mice resulted in multiple mitotic defects and enhanced binucleation. We observed that Aurora B, a critical cytokinetic regulator and a recently identified Chk1 substrate, was mislocalized in mitotic Chk1(+/-) mammary epithelia. Chk1 also exhibited distinct mitotic localization patterns and was active during unperturbed mitosis and cytokinesis in mammalian cells. Active Chk1 expression was not dependent on treatment with spindle poisons such as colcemid during mitosis and cytokinesis. Furthermore, two different complementary approaches demonstrated that abrogation of Chk1 in mitotic mammalian cells resulted in cytokinetic regression and binucleation, increased chromosome lagging and/or nondisjunction, and abnormal localization of Aurora B at late mitotic structures. Thus, Chk1 is a multifunctional kinase that serves as a nexus between the DNA damage response and the mitotic exit pathways during cell-cycle progression to prevent genomic instability and cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.