ArticleThe Journal of cell biology2009
Wac: a new Augmin subunit required for chromosome alignment but not for acentrosomal microtubule assembly in female meiosis.
Article in The Journal of cell biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 68 citations in OpenAlex.
- The role of chromosome/kinetochore-driven microtubule generation in Drosophila spindle assembly.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- How Microtubules Build the Spindle Branch by Branch.Annual review of cell and developmental biology · 2022Review
- The augmin complex architecture reveals structural insights into microtubule branching.Nature communications · 2022Article
- Reconstitution and mechanistic dissection of the human microtubule branching machinery.The Journal of cell biology · 2022Article
- The phospho-docking protein 14-3-3 regulates microtubule-associated proteins in oocytes including the chromosomal passenger Borealin.PLoS genetics · 2022Article
- Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development.eLife · 2021Article
- Spindle assembly and chromosome dynamics during oocyte meiosis.Current opinion in cell biology · 2019Review
- Direct observation of branching MT nucleation in living animal cells.The Journal of cell biology · 2019Article
- A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.Science (New York, N.Y.) · 2019Article
- A computational model of the early stages of acentriolar meiotic spindle assembly.Molecular biology of the cell · 2019Article
- A novel microtubule nucleation pathway for meiotic spindle assembly in oocytes.The Journal of cell biology · 2018Article
- Review
- Female Meiosis: Synapsis, Recombination, and Segregation inGenetics · 2018Review
- Combining microscopy and biochemistry to study meiotic spindle assembly in Drosophila oocytes.Methods in cell biology · 2018Article
- The chromosomal basis of meiotic acentrosomal spindle assembly and function in oocytes.Chromosoma · 2017Review
- Cross-linking mass spectrometry identifies new interfaces of Augmin required to localise the γ-tubulin ring complex to the mitotic spindle.Biology open · 2017Article
- Mitotic spindle assembly in animal cells: a fine balancing act.Nature reviews. Molecular cell biology · 2017Review
- Metaphase Spindle Assembly.Biology · 2017Review
- Kdm5/Lid Regulates Chromosome Architecture in Meiotic Prophase I Independently of Its Histone Demethylase Activity.PLoS genetics · 2016Article
- Non-centrosomal nucleation mediated by augmin organizes microtubules in post-mitotic neurons and controls axonal microtubule polarity.Nature communications · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The bipolar spindle forms without centrosomes naturally in female meiosis and by experimental manipulation in mitosis. Augmin is a recently discovered protein complex required for centrosome-independent microtubule generation within the spindle in Drosophila melanogaster cultured cells. Five subunits of Augmin have been identified so far, but neither their organization within the complex nor their role in developing organisms is known. In this study, we report a new Augmin subunit, wee Augmin component (Wac). Wac directly interacts with another Augmin subunit, Dgt2, via its coiled-coil domain. Wac depletion in cultured cells, especially without functional centrosomes, causes severe defects in spindle assembly. We found that a wac deletion mutant is viable but female sterile and shows only a mild impact on somatic mitosis. Unexpectedly, mutant female meiosis showed robust microtubule assembly of the acentrosomal spindle but frequent chromosome misalignment. For the first time, this study establishes the role of an Augmin subunit in developing organisms and provides an insight into the architecture of the complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.