ArticleMolecular and cellular biology2009
Limiting the extent of the RDN1 heterochromatin domain by a silencing barrier and Sir2 protein levels in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- VirB, a key transcriptional regulator of virulence plasmid genes inJournal of bacteriology · 2021Article
- Article
- tRNAs as a Driving Force of Genome Evolution in Yeast.Frontiers in microbiology · 2021Review
- Depletion of Limiting rDNA Structural Complexes Triggers Chromosomal Instability and Replicative Aging ofGenetics · 2019Article
- tRNA Genes Affect Chromosome Structure and Function via Local Effects.Molecular and cellular biology · 2019Article
- A SIR-independent role for cohesin in subtelomeric silencing and organization.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- RNA Polymerase I and Fob1 contributions to transcriptional silencing at the yeast rDNA locus.Nucleic acids research · 2016Article
- Chromosome boundary elements and regulation of heterochromatin spreading.Cellular and molecular life sciences : CMLS · 2014Review
- Single cell visualization of yeast gene expression shows correlation of epigenetic switching between multiple heterochromatic regions through multiple generations.PLoS biology · 2013Article
- Genome-wide analysis of functional sirtuin chromatin targets in yeast.Genome biology · 2013Article
- Human tRNA genes function as chromatin insulators.The EMBO journal · 2012Article
- A positive but complex association between meiotic double-strand break hotspots and open chromatin in Saccharomyces cerevisiae.Genome research · 2009Article
- TFIIIC bound DNA elements in nuclear organization and insulation.Biochimica et biophysica actaReview
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
In Saccharomyces cerevisiae, transcriptional silencing occurs at the cryptic mating-type loci (HML and HMR), telomeres, and ribosomal DNA (rDNA; RDN1). Silencing in the rDNA is unusual in that polymerase II (Pol II) promoters within RDN1 are repressed by Sir2 but not Sir3 or Sir4. rDNA silencing unidirectionally spreads leftward, but the mechanism of limiting its spreading is unclear. We searched for silencing barriers flanking the left end of RDN1 by using an established assay for detecting barriers to HMR silencing. Unexpectedly, the unique sequence immediately adjacent to RDN1, which overlaps a prominent cohesin binding site (CARL2), did not have appreciable barrier activity. Instead, a fragment located 2.4 kb to the left, containing a tRNA(Gln) gene and the Ty1 long terminal repeat, had robust barrier activity. The barrier activity was dependent on Pol III transcription of tRNA(Gln), the cohesin protein Smc1, and the SAS1 and Gcn5 histone acetyltransferases. The location of the barrier correlates with the detectable limit of rDNA silencing when SIR2 is overexpressed, where it blocks the spreading of rDNA heterochromatin. We propose a model in which normal Sir2 activity results in termination of silencing near the physical rDNA boundary, while tRNA(Gln) blocks silencing from spreading too far when nucleolar Sir2 pools become elevated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.