ArticleMethods in enzymology2009
Model membrane thermodynamics and lateral distribution of cholesterol: from experimental data to Monte Carlo simulation.
Article in Methods in enzymology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Does Complexation of Plasma Membrane Cholesterol with Phospholipids Determine its Transbilayer Distribution?The Journal of membrane biology · 2026Article
- A Membrane-Assisted Mechanism for the Release of Ceramide from the CERT START Domain.The journal of physical chemistry. B · 2024Article
- Cholesterol impacts the formation of huntingtin/lipid complexes and subsequent aggregation.Protein science : a publication of the Protein Society · 2023Article
- Pneumolysin-damaged cells benefit from non-homogeneous toxin binding to cholesterol-rich membrane domains.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2018Article
- Membrane interaction of retroviral Gag proteins.Frontiers in microbiology · 2014Review
- Comparative computer simulation study of cholesterol in hydrated unary and binary lipid bilayers and in an anhydrous crystal.The journal of physical chemistry. B · 2013Article
- Large conductance, calcium- and voltage-gated potassium (BK) channels: regulation by cholesterol.Pharmacology & therapeutics · 2012Review
- Cholesterol superlattice modulates CA4P release from liposomes and CA4P cytotoxicity on mammary cancer cells.Biophysical journal · 2012Article
- Modification of Lipid Bilayer Structure by Diacylglycerol: A Comparative Study of Diacylglycerol and Cholesterol.Journal of chemical theory and computation · 2012Article
- Simulation of the lo-ld phase boundary in DSPC/DOPC/cholesterol ternary mixtures using pairwise interactions.The journal of physical chemistry. B · 2011Article
- Orientation of tie-lines in the phase diagram of DOPC/DPPC/cholesterol model biomembranes.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- A molecular view of the cholesterol condensing effect in DOPC lipid bilayers.The journal of physical chemistry. B · 2010Article
- Instability of cholesterol clusters in lipid bilayers and the cholesterol's Umbrella effect.The journal of physical chemistry. B · 2010Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Thermodynamic analysis and Monte Carlo simulation techniques were used to study cholesterol-lipid interactions in lipid membranes. Experimental data, including the maximum solubility of cholesterol in lipid bilayers, the 1-to-1 displacement of cholesterol by ceramide, and the cholesterol chemical activity with cholesterol oxidase (COD), were systematically analyzed using thermodynamic principles. A conceptual model, the umbrella model, is presented to describe the key cholesterol-lipid interaction in lipid membranes. In a lipid membrane, nonpolar cholesterol relies on polar phospholipid headgroup coverage to avoid the unfavorable free energy of cholesterol contact with water. This coverage requirement leads to cholesterol's strong tendency not to clustering in a bilayer, its preferential association with large headgroup lipids with saturated acyl chains, and its competition with ceramide for large headgroup lipids. The umbrella model was parameterized into a form of multibody (i.e., nonpairwise) interaction for Monte Carlo simulation, and the measured chemical potentials of cholesterol agreed favorably with the predictions from the simulation. Under the right conditions, the multibody interactions can also lead to the formation of cholesterol superlattices. Also, an intrinsic thermodynamic connection between a jump in chemical potential and a regular distribution (RD) of membrane molecules was uncovered. This study shows that combining thermodynamics with computer simulation can be a productive approach for analyzing and interpreting complex experimental data, and thermodynamics can yield a predicting power in bioscience research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.