ArticleBiophysical journal2009
Uniform action potential repolarization within the sarcolemma of in situ ventricular cardiomyocytes.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Guidelines for assessment of cardiac electrophysiology and arrhythmias in small animals.American journal of physiology. Heart and circulatory physiology · 2022Review
- Primer to Voltage Imaging With ANNINE Dyes and Two-Photon Microscopy.Frontiers in cellular neuroscience · 2019Review
- Electrical coupling between ventricular myocytes and myofibroblasts in the infarcted mouse heart.Cardiovascular research · 2018Article
- Electrical defects of the transverse-axial tubular system in cardiac diseases.The Journal of physiology · 2017Review
- Article
- Reciprocal Modulation of IFrontiers in physiology · 2016Article
- High-resolution non-contact measurement of the electrical activity of plants in situ using optical recording.Scientific reports · 2015Article
- In situ single photon confocal imaging of cardiomyocyte T-tubule system from Langendorff-perfused hearts.Frontiers in physiology · 2015Review
- Mechanosensitive kinases regulate stiffness-induced cardiomyocyte maturation.Scientific reports · 2014Article
- Intravital imaging of cardiac function at the single-cell level.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging.Journal of neuroscience methods · 2014Article
- Functional cardiac imaging by random access microscopy.Frontiers in physiology · 2014Article
- Micron-scale voltage and [Ca(2+)]i imaging in the intact heart.Frontiers in physiology · 2014Review
- Non-uniform distribution of outer hair cell transmembrane potential induced by extracellular electric field.Biophysical journal · 2013Article
- The transverse-axial tubular system of cardiomyocytes.Cellular and molecular life sciences : CMLS · 2013Review
- Spatial variability in T-tubule and electrical remodeling of left ventricular epicardium in mouse hearts with transgenic Gαq overexpression-induced pathological hypertrophy.Journal of molecular and cellular cardiology · 2012Article
- Stimulated emission depletion live-cell super-resolution imaging shows proliferative remodeling of T-tubule membrane structures after myocardial infarction.Circulation research · 2012Article
- In situ confocal imaging in intact heart reveals stress-induced Ca(2+) release variability in a murine catecholaminergic polymorphic ventricular tachycardia model of type 2 ryanodine receptor(R4496C+/-) mutation.Circulation. Arrhythmia and electrophysiology · 2012Article
- Action potential propagation in transverse-axial tubular system is impaired in heart failure.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Metabolic stress in isolated mouse ventricular myocytes leads to remodeling of t tubules.American journal of physiology. Heart and circulatory physiology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Previous studies have speculated, based on indirect evidence, that the action potential at the transverse (t)-tubules is longer than at the surface membrane in mammalian ventricular cardiomyocytes. To date, no technique has enabled recording of electrical activity selectively at the t-tubules to directly examine this hypothesis. We used confocal line-scan imaging in conjunction with the fast response voltage-sensitive dyes ANNINE-6 and ANNINE-6plus to resolve action potential-related changes in fractional dye fluorescence (DeltaF/F) at the t-tubule and surface membranes of in situ mouse ventricular cardiomyocytes. Peak DeltaF/F during action potential phase 0 depolarization averaged -21% for both dyes. The shape and time course of optical action potentials measured with the water-soluble ANNINE-6plus were indistinguishable from those of action potentials recorded with intracellular microelectrodes in the absence of the dye. In contrast, optical action potentials measured with the water-insoluble ANNINE-6 were significantly prolonged compared to the electrical recordings obtained from dye-free hearts, suggesting electrophysiological effects of ANNINE-6 and/or its solvents. With either dye, the kinetics of action potential-dependent changes in DeltaF/F during repolarization were found to be similar at the t-tubular and surface membranes. This study provides what to our knowledge are the first direct measurements of t-tubule electrical activity in ventricular cardiomyocytes, which support the concept that action potential duration is uniform throughout the sarcolemma of individual cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.