ArticleBiophysical journal2009
Role of E-cadherin in membrane-cortex interaction probed by nanotube extrusion.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- Modulation of E-Cadherin Function through the AmotL2 Isoforms Promotes Ameboid Cell Invasion.Cells · 2023Article
- Adhesion strength between cells regulate nonmonotonic growth by a biomechanical feedback mechanism.Biophysical journal · 2022Article
- Tissue Regeneration from Mechanical Stretching of Cell-Cell Adhesion.Tissue engineering. Part C, Methods · 2019Review
- Signaling Pathways Induced by Leptin during Epithelial⁻Mesenchymal Transition in Breast Cancer.International journal of molecular sciences · 2018Review
- Src- and confinement-dependent FAK activation causes E-cadherin relaxation and β-catenin activity.The Journal of cell biology · 2018Article
- Non-equilibrium Membrane Homeostasis in Expanding Cellular Domains.Biophysical journal · 2017Article
- Article
- Mechanics of Biomimetic Liposomes Encapsulating an Actin Shell.Biophysical journal · 2015Article
- Regional-specific alterations in cell-cell junctions, cytoskeletal networks and myosin-mediated mechanical cues coordinate collectivity of movement of epithelial cells in response to injury.Experimental cell research · 2014Article
- Three functions of cadherins in cell adhesion.Current biology : CB · 2013Review
- E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- E-cadherin-dependent stimulation of traction force at focal adhesions via the Src and PI3K signaling pathways.Biophysical journal · 2012Article
- The role of adhesion energy in controlling cell-cell contacts.Current opinion in cell biology · 2011Review
- Membrane invaginations reveal cortical sites that pull on mitotic spindles in one-cell C. elegans embryos.PloS one · 2010Article
- Biophysical mechanisms of single-cell interactions with microtopographical cues.Biomedical microdevices · 2010Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study aims to define the role of E-cadherin (Ecad) engagement in cell-cell contact during membrane-cortex interaction. As a tool, we used a hydrodynamic membrane tube extrusion technique to characterize the mechanical interaction between the plasma membrane and the underlying cortical cytoskeleton. Cells were anchored on 4.5 microm beads coated with polylysine (PL) to obtain nonspecific cell adhesion or with an antibody against Ecad to mimic specific Ecad-mediated cell adhesion. We investigated tube length dynamics L(t) over time and through successive extrusions applied to the cell at regular time intervals. A constant slow velocity was observed for the first extrusion, for PL-attached cells. Subsequent extrusions had two phases: an initial high-velocity regime followed by a low-velocity regime. Successive extrusions gradually weakened the binding of the membrane around the tube neck to the underlying cortical cytoskeleton. Cells specifically attached via Ecad first exhibited a very low extrusion velocity regime followed by a faster extrusion regime similar to nonspecific extrusion. This indicates that Ecad strengthens the membrane-cortical cytoskeleton interaction, but only in a restricted area corresponding to the site of contact between the cell and the bead. Occasional giant "cortex" tubes were extruded with specifically anchored cells, demonstrating that the cortex remained tightly bound to the membrane through Ecad-mediated adhesion at the contact site.
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Registered trials
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